Basigin/CD147 Protein, Human (Biotinylated, HEK293, Avi-His)

Basigin/BSG proteins are critical for retinal maturation and development and function as retinal cell receptors for NXNL1, promoting the survival of retinal cone photoreceptors. Basigin/CD147 Protein, Human (Biotinylated, HEK293, Avi-His) is the recombinant human-derived Basigin/CD147 protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag.

Basigin/BSG protein is essential for the normal maturation and development of the retina, functioning as a critical cell surface receptor for NXNL1 and playing a pivotal role in NXNL1-mediated survival of retinal cone photoreceptors. Collaborating with glucose transporter SLC16A1/GLUT1 and NXNL1, Basigin/BSG promotes the survival of retinal cones by facilitating aerobic glycolysis and accelerating glucose entry into photoreceptors. Additionally, it serves as a potent inducer of IL6 secretion in various cell lines, including monocytes. In the context of microbial infection, Basigin/BSG acts as an erythrocyte receptor for P. falciparum RH5, playing an indispensable role in erythrocyte invasion by the merozoite stage of P. falciparum isolates 3D7 and Dd2. These diverse functions highlight the multifaceted roles of Basigin/BSG in cellular processes and host-pathogen interactions.

Human

HEK293

C-Avi;C-6*His

P35613-2 (A22-H205)

682  [NCBI]

Partial

Biotin

The single lysine residue in Avitag is biotinylated through an enzymatic reaction.

AAGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSH

30-45 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/nci-h209-cell.html