INPP5A Protein, Human (HEK293, His)

INPP5A Protein, a phosphatase, catalyzes the hydrolysis of inositol phosphates, converting inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrasphosphate to inositol 1,3,4-trisphosphate. This enzymatic action underscores its role in inositol phosphate metabolism. INPP5A is crucial for the survival of cerebellar Purkinje cells, emphasizing its significance in cellular processes and neuronal function. INPP5A Protein, Human (HEK293, His) is the recombinant human-derived INPP5A protein, expressed by HEK293 , with C-6*His labeled tag.

INPP5A Protein is a phosphatase with a specific enzymatic activity, catalyzing the hydrolysis of the 5-phosphate of inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate, and inositol 1,3,4,5-tetrasphosphate to inositol 1,3,4-trisphosphate. This enzymatic action highlights its role in inositol phosphate metabolism. Notably, INPP5A plays a crucial and essential role in the survival of cerebellar Purkinje cells, emphasizing its significance in cellular processes and neuronal function.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

Q14642 (M1-V410)

3632  [NCBI]

Partial

MAGKAAAPGTAVLLVTANVGSLFDDPENLQKNWLREFYQVVHTHKPHFMALHCQEFGGKNYEASMSHVDKFVKELLSSDAMKEYNRARVYLDENYKSQEHFTALGSFYFLHESLKNIYQFDFKAKKYRKVAGKEIYSDTLESTPMLEKEKFPQDYFPECKWSRKGFIRTRWCIADCAFDLVNIHLFHDASNLVAWETSPSVYSGIRHKALGYVLDRIIDQRFEKVSYFVFGDFNFRLDSKSVVETLCTKATMQTVRAADTNEVVKLIFRESDNDRKVMLQLEKKLFDYFNQEVFRDNNGTALLEFDKELSVFKDRLYELDISFPPSYPYSEDARQGEQYMNTRCPAWCDRILMSPSAKELVLRSESEEKVVTYDHIGPNVCMGDHKPVFLAFRIMPGAGKPHAHVHKCCV

Approximately 45 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Solution

1.Supplied as a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
2.Supplied as a 0.22 μm filtered solution in 500 mM Tris-HCl, 10 mM MgCl₂, 100 mM KCl, pH 7.5.
3.Supplied as a 0.22 μm filtered solution in 50 mM Tris-HCl, 10 mM MgCl₂, 100 mM KCl, pH 7.5.
Please refer to the lot-specific COA for specific buffer information.
Note: If this product is used in SPR assay, please note that the protein buffer should not contain primary amino components (such as Tris and Imidazole), and the buffer needs to be replaced.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. /biology-dictionary/b16-f10-cell.html  [Content Brief]