LILRB4/CD85k/ILT3 Protein, Human (Biotinylated, HEK293, His-Avi)

The LILRB4/CD85k/ILT3 protein is a key inhibitory receptor that plays a key role in immune regulation and tolerance. As a receptor for FN1, apolipoprotein APOE, and ALCAM/CD166, it inhibits receptor-mediated phosphorylation, intracellular calcium mobilization, and FCGR1A/CD64-mediated monocyte activation, thereby reducing TNF production. LILRB4/CD85k/ILT3 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived LILRB4/CD85k/ILT3 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

LILRB4/CD85k/ILT3, an inhibitory receptor, plays a pivotal role in immune regulation and the establishment of immune tolerance. Functioning as a receptor for FN1, apolipoprotein APOE, and ALCAM/CD166, this protein is involved in diverse cellular processes. It inhibits receptor-mediated phosphorylation of cellular proteins and the mobilization of intracellular calcium ions, and it further down-regulates FCGR1A/CD64-mediated monocyte activation, leading to reduced TNF production. Additionally, LILRB4/ILT3 impedes T cell proliferation, inducing anergy, suppressing the differentiation of IFNG-producing CD8+ cytotoxic T cells, and promoting the generation of CD8+ T suppressor cells. It induces the up-regulation of CD86 on dendritic cells and interferes with TNFRSF5-signaling and NF-kappa-B up-regulation. The inhibitory effects are at least partially mediated through interactions with FN1 and the phosphatase PTPN6.

1.Measured by its binding ability in a functional ELISA. When immobilized Anti-LILRB4 Antibody at 1μg/ml (100μl/Well), can bind Biotinylated Human LILRB4 and the EC₅₀ is < 10 ng/mL.
2.Immobilized Biotinylated Human LILRB4, His Avi Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-LILRB4 Antibody, hFc Tag with the EC₅₀ of 1.0 μg/ml determined by ELISA.

Human

HEK293

C-Avi;C-6*His

Q8NHJ6-1 (Q22-H257)

11006  [NCBI]

Extracellular Domain

Biotin

QAGPLPKPTLWAEPGSVISWGNSVTIWCQGTLEAREYRLDKEESPAPWDRQNPLEPKNKARFSIPSMTEDYAGRYRCYYRSPVGWSQPSDPLELVMTGAYSKPTLSALPSPLVTSGKSVTLLCQSRSPMDTFLLIKERAAHPLLHLRSEHGAQQHQAEFPMSPVTSVHGGTYRCFSSHGFSHYLLSHPSDPLELIVSGSLEDPRPSPTRSVSTAAGPEDQPLMPTGSVPHSGLRRH

Approximately 38-42 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by Bis-Tris PAGE.

Lyophilized powder.

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/baf3-cell.html  [Content Brief]