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BMP-2 Protein, Human/Mouse/Rat

21 Cited Publications
Customer Review

Based on 21 publication(s) in Google Scholar

Bone morphogenetic protein 2 (BMP-2) is a pleiotropic ligand protein belonging to TNFβ family, and is involved in key embryonic development of vascular and valvular homeostasis. BMP-2 binds to type I receptors (ALK-2/-3/-6) and type II receptors (BMPR2, ACVR2A) to regulate various types of calcification, including atherosclerosis, chronic kidney disease, diabetes, and valve calcification. BMP-2 is overexpressed by myofibroblast and preosteoblast in the calcified area of human calcified valve, which are densely infiltrated by B lymphocytes and T lymphocytes. BMP-2 is the junction between atherosclerotic vascular calcification and normal bone formation mechanism. BMP-2 Protein, Human/Mouse/Rat is 114 a.a. (Q283-R396), expressed in E. coli.

For research use only. We do not sell to patients.
  • Species: Rat; Mouse; Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for BMP-2 Protein, Human/Mouse/Rat
  • Experimental Validation Results for BMP-2 Protein, Human/Mouse/Rat
  • Experimental Validation Results for BMP-2 Protein, Human/Mouse/Rat
  • Experimental Validation Results for BMP-2 Protein, Human/Mouse/Rat
  • Experimental Validation Results for BMP-2 Protein, Human/Mouse/Rat
  • BMP-2 Protein, Human/Mouse/Rat Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

Bone morphogenetic protein 2 (BMP-2) is a pleiotropic ligand protein belonging to TNFβ family, and is involved in key embryonic development of vascular and valvular homeostasis. BMP-2 binds to type I receptors (ALK-2/-3/-6) and type II receptors (BMPR2, ACVR2A) to regulate various types of calcification, including atherosclerosis, chronic kidney disease, diabetes, and valve calcification[1]. BMP-2 is overexpressed by myofibroblast and preosteoblast in the calcified area of human calcified valve, which are densely infiltrated by B lymphocytes and T lymphocytes[2]. BMP-2 is the junction between atherosclerotic vascular calcification and normal bone formation mechanism[3]. BMP-2 Protein, Human/Mouse/Rat is 114 a.a. (Q283-R396), expressed in E. coli.

Background

Bone Morphogenetic Protein 2 (BMP-2) is a ligand protein with pleiotropic, belongs to TNFβ family. BMP-2 formats BMP/TGFβ signaling to involve in vascular and valvular homeostasis, which is a critical process of embryonic development[1].
BMP-2/TGFβ signaling can be terminated by inhibitory SMADs including SMAD6 and SMAD7, which are activated and induced by BMP signaling and switch off BMP signaling via multiple mechanisms[4].
BMP-2 is widely found in different animals, while the sequence in human is similar to Rat (91.86%), and mouse (92.13%).
BMPs exhibits critical contributions to the pathophysiology of atherosclerosis, pulmonary vascular disease, and vascular and valvular calcification[1].
BMP-2 binds different receptor, such as type I receptors (ALK-2/-3/-6) and type II receptors (BMPR2, ACVR2A), to regulate various calcification type including Atherosclerosis, Chronic Kidney Disease, Diabetes, Valvular Calcification[1].
BMP-2 promotes monocyte infiltration and inflammation of atherosclerotic legions[5].
It is linked to increased plaque formation via pro-inflammatory and pro-atherogenic effects, promoting oxidative stress, endothelial dysfunction and osteogenic differentiation[6].
BMP-2 is overexpressed in ossified regions of human calcified valves by myofibroblasts and pre-osteoblasts in areas densely infiltrated with B- and T-lymphocytes[2].
And it serves as the linkers between atherosclerotic vascular calcification with mechanisms of normal bone formation[3].
BMP-2 induces angiogenesis, endothelial cells (ECs) proliferation, and migration[7].
And BMP-2 also enhances the expression of the osteoblast and chondrocyte master transcriptional regulator RUNX2 to promote the mineralization of cultured human coronary vascular SMCs in a manner that was dependent on oxidative stress and endoplasmic reticulum (ER) stress[8].

In Vitro

BMP-2 (10 nM; 15 min) shows little effect on the phosphorylation of MADR1, indicating MADR1 is a downstream component in the BMP2 signal transduction pathway[9].
BMP-2 (50 ng/mL; 14 d) induces osteogenic differentiation of mesenchymal stem cells (MSCs) as well as strong synergistic effect with 1 ng/mL VEGF and 10 ng/mL bFGF, in bone marrow of femurs and tibias of 6 week-old rats[10].

Verified Bioactivity

1. Measured by its ability to induce alkaline phosphatase production by ATDC-5 Cells. The ED50 for this effect is typically <0.2 µg/mL.
2. Measured by its ability to induce alkaline phosphatase production by C2C12 cells. The ED50 for this effect is typically <1 µg/mL.

Technical Parameters

  • Species Rat; Mouse; Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    P12643 (Q283-R396)

  • Gene ID
    650  [NCBI]
  • Molecular Construction
    • N-term
    • BMP-2 (Q283-R396)
      Accession # P12643
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    BMP2; SSFSC1; Prev. BMP2A; SSFSC; Bone Morphogenetic Protein 2A; BMP-2; BMP2 Protein; BDA2; Bone Morphogenetic Protein 2; BMP-2A

  • AA Sequence

    QAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR

  • Molecular Weight

    Approximately 13 kDa under reduced (R) conditions, 26 kDa under non reducing (N) conditions, based on SDS-PAGE.

  • Structure/Form

    Homodimer

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 50 mM acetic acid.
2.Lyophilized from a 0.22 μm filtered solution of 50 mM acetic acid, pH 4.0, 5% mannitol.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in 20 mM HAc. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Yang P, et al. The role of bone morphogenetic protein signaling in vascular calcification. Bone. 2020 Dec;141:115542. [Content Brief]

[2]. Miyazawa K, et al. Regulation of TGF-β Family Signaling by Inhibitory Smads. Cold Spring Harb Perspect Biol. 2017 Mar 1;9(3):a022095. [Content Brief]

[3]. Simões Sato AY, et al. BMP-2 and -4 produced by vascular smooth muscle cells from atherosclerotic lesions induce monocyte chemotaxis through direct BMPRII activation. Atherosclerosis. 2014 Jul;235(1):45-55. [Content Brief]

[4]. Boström K, et al. Bone morphogenetic protein expression in human atherosclerotic lesions. J Clin Invest. 1993 Apr;91(4):1800-9. [Content Brief]

[5]. Mohler ER 3rd, et al. Bone formation and inflammation in cardiac valves. Circulation. 2001 Mar 20;103(11):1522-8. [Content Brief]

[6]. Demer LL, et al. Mechanism of calcification in atherosclerosis. Trends Cardiovasc Med. 1994 Jan-Feb;4(1):45-9. [Content Brief]

[7]. David L, et al. Emerging role of bone morphogenetic proteins in angiogenesis. Cytokine Growth Factor Rev. 2009 Jun;20(3):203-12. [Content Brief]

[8]. Liberman M, et al. Bone morphogenetic protein-2 activates NADPH oxidase to increase endoplasmic reticulum stress and human coronary artery smooth muscle cell calcification. Biochem Biophys Res Commun. 2011 Sep 30;413(3):436-41. [Content Brief]

[9]. Hoodless PA, et al. MADR1, a MAD-related protein that functions in BMP2 signaling pathways. Cell. 1996 May 17;85(4):489-500. [Content Brief]

[10]. Bai Y, et al. BMP-2, VEGF and bFGF synergistically promote the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells. Biotechnol Lett. 2013 Mar;35(3):301-8. [Content Brief]

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Calculators

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
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= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
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MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    BMP-2 Protein, Human/Mouse/Rat purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Dec 9;26(24):11858. [Abstract]

    Customer Validation
     Expression patterns of key chondrocyte differentiation-related genes in ATDC5 cells following T-2 toxin and BMP2 (100 ng/mL; 48 h) recombinant protein intervention were analyzed.

  • 1/16

    BMP-2 Protein, Human/Mouse/Rat purchased from MedChemExpress. Usage Cited in: Cell. 2024 Oct 17;187(21):6016-6034.e25. [Abstract]

    Customer Validation
     Immunoblot for recombinant BMP2 (rBMP2) treatment in melanoma lines at indicated concentrations (for 30 minutes) showed changes in protein expression.

  • 1/16

    BMP-2 Protein, Human/Mouse/Rat purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Mar;11(10):e2308072. [Abstract]

    Customer Validation
     The immunofluorescence of pSmad1/5/8 was assessed 24 h after photostimulation, BMP2 (100 ng/mL; 24 h) treated (n = 16 fields in 3 independent trials for each group), with or without BMPR-I inhibitor LDN193189 (1 μM) and BMP ligand antagonist noggin (100 ng/mL), compared with the control group.

  • 1/16

    BMP-2 Protein, Human/Mouse/Rat purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Mar;11(10):e2308072. [Abstract]

    Customer Validation
     The phosphorylated Smad 2 (pSmad2) expression level in ADSCs 3 h and 24 h after photostimulation, BMP2 (100 ng/mL) and DEX (10 nM) treated (n = 16 fields in 3 independent trials for each group) was measured.

  • 1/16

    BMP-2 Protein, Human/Mouse/Rat purchased from MedChemExpress. Usage Cited in: Biosci Rep. 2019 Sep 24;39(9):BSR20191405. [Abstract]

    Customer Validation
     Determination of the expression of miR-128 in C2C12 cells treated with BMP-2 (2 nM) at days 0, 5, 10, and 21 by qRT-PCR was performed.

  • BMP-2 Protein, Human/Mouse/Rat Product Image
    1/16
  • Experimental Validation Results for BMP-2 Protein, Human/Mouse/Rat
  • Experimental Validation Results for BMP-2 Protein, Human/Mouse/Rat
  • Experimental Validation Results for BMP-2 Protein, Human/Mouse/Rat
  • Experimental Validation Results for BMP-2 Protein, Human/Mouse/Rat
  • Experimental Validation Results for BMP-2 Protein, Human/Mouse/Rat
  • BMP-2 Protein, Human/Mouse/Rat Product Image

  • BMP-2 Protein, Human/Mouse/Rat purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Dec 9;26(24):11858. [Abstract]

    Expression patterns of key chondrocyte differentiation-related genes in ATDC5 cells following T-2 toxin and BMP2 (100 ng/mL; 48 h) recombinant protein intervention were analyzed.

  • BMP-2 Protein, Human/Mouse/Rat purchased from MedChemExpress. Usage Cited in: Cell. 2024 Oct 17;187(21):6016-6034.e25. [Abstract]

    Immunoblot for recombinant BMP2 (rBMP2) treatment in melanoma lines at indicated concentrations (for 30 minutes) showed changes in protein expression.

  • BMP-2 Protein, Human/Mouse/Rat purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Mar;11(10):e2308072. [Abstract]

    The immunofluorescence of pSmad1/5/8 was assessed 24 h after photostimulation, BMP2 (100 ng/mL; 24 h) treated (n = 16 fields in 3 independent trials for each group), with or without BMPR-I inhibitor LDN193189 (1 μM) and BMP ligand antagonist noggin (100 ng/mL), compared with the control group.

  • BMP-2 Protein, Human/Mouse/Rat purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Mar;11(10):e2308072. [Abstract]

    The phosphorylated Smad 2 (pSmad2) expression level in ADSCs 3 h and 24 h after photostimulation, BMP2 (100 ng/mL) and DEX (10 nM) treated (n = 16 fields in 3 independent trials for each group) was measured.

  • BMP-2 Protein, Human/Mouse/Rat purchased from MedChemExpress. Usage Cited in: Biosci Rep. 2019 Sep 24;39(9):BSR20191405. [Abstract]

    Determination of the expression of miR-128 in C2C12 cells treated with BMP-2 (2 nM) at days 0, 5, 10, and 21 by qRT-PCR was performed.