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MCP-3/CCL7 Protein, Mouse

5 Cited Publications
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Based on 5 publication(s) in Google Scholar

MCP-3/CCL7 Protein, Mouse is a CC chemokine and elicitor that binds to CCR1, CCR2 and CCR3 to mediate antiviral, antibacterial, antitumor and other immune responses. MCP-3/CCL7 Protein, Mouse is a recombinant mouse MCP-3/CCL7 protein expressed by E.coilMCP-3/CCL7(Q24-P97).

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for MCP-3/CCL7 Protein, Mouse
  • Experimental Validation Results for MCP-3/CCL7 Protein, Mouse
  • Experimental Validation Results for MCP-3/CCL7 Protein, Mouse
  • Experimental Validation Results for MCP-3/CCL7 Protein, Mouse
  • Experimental Validation Results for MCP-3/CCL7 Protein, Mouse
  • Experimental Validation Results for MCP-3/CCL7 Protein, Mouse
  • Experimental Validation Results for MCP-3/CCL7 Protein, Mouse
  • MCP-3/CCL7 Protein, Mouse Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

MCP-3/CCL7 Protein, Mouse is a CC chemokine and elicitor that binds to CCR1, CCR2 and CCR3 to mediate antiviral, antibacterial, antitumor and other immune responses. MCP-3/CCL7 Protein, Mouse is a recombinant mouse MCP-3/CCL7 protein expressed by E.coilMCP-3/CCL7(Q24-P97)[1].

Background

CCL7, also known as monocyte chemotactic protein 3 (MCP3), is a small cell factor. In the human genome, CCL7 is encoded by the CCL7 gene located on chromosome 17q11.2-q12. It consists of 99 amino acids, including a signal peptide of 23 amino acids, while a mature protein of approximately 76 amino acids is secreted upon signal peptide cleavage. CCL7 is expressed in a variety of cell types, such as stromal cells, keratin-forming cells, airway smooth muscle cells, parenchymal cells, fibroblasts and leukocytes, and tumor cells[1]. CCL7 is generally present as a monomer and can bind to a variety of receptors, including CCR1, CCR2, CCR3, CCR5 and CCR10 to mediate effects on immune cell types.CCL7 can act as a chemoattractant, attracting a variety of leukocytes, including monocytes and neutrophils. It mediates the immune response by recruiting leukocytes to infected tissues and is also involved in monocyte mobilization and recruitment of monocytes to sites of inflammation, as well as inducing neutrophil migration to sites of inflammation by increasing intracellular Ca2+ flux. CCL7 is involved in antibacterial, antiviral and antifungal immune responses, as well as being associated with various immune diseases such as ulcerative colitis, multiple sclerosis or non-atopic and atopic asthma. At the same time, CCL7 expression activates antitumor immune responses[2].

In Vitro

CCL7(100 ng/mL, 8 h) reduces the fertilization rate of oocytes and sperm to 31.1 % in cumulus-oocyte complex (COC) but does not affect the fertilization rate of oocytes without oocula[3].

In Vivo

CCL7(i.p., 50 μg/kg) does not significantly alter ovulation, but decreases fertilization rate and significantly stimulates sperm chemotaxis without affecting spermatozoa chemotaxis in wide female mice[3].

Verified Bioactivity

1.Full biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 100-300 ng/ml.
2.Measured by its ability to chemoattract THP-1 human monocytes. The ED50 this effect is 153.9 ng/mL, corresponding to a specific activity is 6497.726 U/mg.
3.Determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR2A.The ED50 for this effect is 0.7142 μg/mL, corresponding to a specific activity is 1400.168 U/mg.

Technical Parameters

  • Species Mouse
  • Source E. coli
  • Tag Tag Free
  • Accession

    Q03366 (Q24-P97)

  • Gene ID
    20306  [NCBI]
  • Molecular Construction
    • N-term
    • CCL7 (Q24-P97)
      Accession # Q03366
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    CCL7; Monocyte Chemotactic Protein 3; Prev. SCYA6; FIC; Prev. SCYA7; Small Inducible Cytokine A7 (Monocyte Chemotactic Protein 3); MCP-3; Chemokine (C-C Motif) Ligand 7; NC28; Small-Inducible Cytokine A7; MCP3; C-C Motif Chemokine 7; Monocyte Chemoattract

  • AA Sequence

    QPDGPNASTCCYVKKQKIPKRNLKSYRRITSSRCPWEAVIFKTKKGMEVCAEAHQKWVEEAIAYLDMKTPTPKP

  • Predicted Molecular Mass

    8.5 kDa

  • Molecular Weight

    Approximately 12 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 2×PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 40 mM PB, 300 mM NaCl, pH 7.4.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. G Opdenakker, et al. The human MCP-3 gene (SCYA7): cloning, sequence analysis, and assignment to the C-C chemokine gene cluster on chromosome 17q11.2-q12. Genomics. 1994 May 15;21(2):403-8. [Content Brief]

[2]. F Fioretti, et al. Reduced tumorigenicity and augmented leukocyte infiltration after monocyte chemotactic protein-3 (MCP-3) gene transfer: perivascular accumulation of dendritic cells in peritumoral tissue and neutrophil recruitment within the tumor. J Immunol. 1998 Jul 1;161(1):342-6. [Content Brief]

[3]. Shigero Tamba, et al. Timely interaction between prostaglandin and chemokine signaling is a prerequisite for successful fertilization. Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14539-44. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to chemoattract THP-1 human monocytes. The ED50 for this effect is 153.9 ng/mL, corresponding to a specific activity is 6497.726 U/mg.

  • 1/16

    MCP-3/CCL7 Protein, Mouse purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2026 Feb 1:170:116059. [Abstract]

    Customer Validation
     TBHQ reduced the hyperalgesia ratio in mice treated with either ISL or CCR2 KD following intraplantar CCL7 (MCP-3/CCL7 Protein, Mouse, 100 ng, sc) injection.

  • 1/16

    MCP-3/CCL7 Protein, Mouse purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Dec 22;16(1):918. [Abstract]

    Customer Validation
     Schematic illustration of the experimental design showing orthotopic implantation of MOC1 cells in WT and Zbp1−/− mice, along with intraperitoneal injections of BX471 and recombinant CCL7 (MCP-3/CCL7 Protein, Mouse, 100 μg/kg, ip , daily, 3 weeks).

  • 1/16

    MCP-3/CCL7 Protein, Mouse purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Dec 22;16(1):918. [Abstract]

    Customer Validation
     Tumor formation in four groups: WT, WT + BX471, Zbp1−/−, and Zbp1−/−+CCL7 (MCP-3/CCL7 Protein, Mouse, 100 μg/kg, ip , daily, 3 weeks).

  • 1/16

    MCP-3/CCL7 Protein, Mouse purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Dec 22;16(1):918. [Abstract]

    Customer Validation
     CCL7 (MCP-3/CCL7 Protein, Mouse, 100 μg/kg, ip , daily, 3 weeks) administration reversed the inhibitory effect of BX471 on Zbp1−/− tumors.

  • 1/16

    MCP-3/CCL7 Protein, Mouse purchased from MedChemExpress. Usage Cited in: Mol Psychiatry. 2025 May;30(5):2137-2151. [Abstract]

    Customer Validation
     The NOLT following microinjections of Clo-lip and CCL7 (MCP-3/CCL7 Protein, Mouse, 60 ng/μL/0.5 μL/side). Exploratory preference to object at the novel location in the test session (right) was measured 24 h following the training session (left).

  • MCP-3/CCL7 Protein, Mouse Product Image
    1/16
  • Experimental Validation Results for MCP-3/CCL7 Protein, Mouse
  • Experimental Validation Results for MCP-3/CCL7 Protein, Mouse
  • Experimental Validation Results for MCP-3/CCL7 Protein, Mouse
  • Experimental Validation Results for MCP-3/CCL7 Protein, Mouse
  • Experimental Validation Results for MCP-3/CCL7 Protein, Mouse
  • Experimental Validation Results for MCP-3/CCL7 Protein, Mouse
  • Experimental Validation Results for MCP-3/CCL7 Protein, Mouse
  • MCP-3/CCL7 Protein, Mouse Product Image

  • MCP-3/CCL7 Protein, Mouse purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2026 Feb 1:170:116059. [Abstract]

    TBHQ reduced the hyperalgesia ratio in mice treated with either ISL or CCR2 KD following intraplantar CCL7 (MCP-3/CCL7 Protein, Mouse, 100 ng, sc) injection.

  • MCP-3/CCL7 Protein, Mouse purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Dec 22;16(1):918. [Abstract]

    Schematic illustration of the experimental design showing orthotopic implantation of MOC1 cells in WT and Zbp1−/− mice, along with intraperitoneal injections of BX471 and recombinant CCL7 (MCP-3/CCL7 Protein, Mouse, 100 μg/kg, ip , daily, 3 weeks).

  • MCP-3/CCL7 Protein, Mouse purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Dec 22;16(1):918. [Abstract]

    Tumor formation in four groups: WT, WT + BX471, Zbp1−/−, and Zbp1−/−+CCL7 (MCP-3/CCL7 Protein, Mouse, 100 μg/kg, ip , daily, 3 weeks).

  • MCP-3/CCL7 Protein, Mouse purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Dec 22;16(1):918. [Abstract]

    CCL7 (MCP-3/CCL7 Protein, Mouse, 100 μg/kg, ip , daily, 3 weeks) administration reversed the inhibitory effect of BX471 on Zbp1−/− tumors.

  • MCP-3/CCL7 Protein, Mouse purchased from MedChemExpress. Usage Cited in: Mol Psychiatry. 2025 May;30(5):2137-2151. [Abstract]

    The NOLT following microinjections of Clo-lip and CCL7 (MCP-3/CCL7 Protein, Mouse, 60 ng/μL/0.5 μL/side). Exploratory preference to object at the novel location in the test session (right) was measured 24 h following the training session (left).