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  3. D-Val-Leu-Lys-AMC TFA

D-Val-Leu-Lys-AMC TFA is a selective fluorogenic peptide substrate of plasmin. D-Val-Leu-Lys-AMC TFA can be used for the quantification of enzymatic activity of plasmin. Ex: 360-380 nm, Em: 440-460 nm.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

D-Val-Leu-Lys-AMC TFA

D-Val-Leu-Lys-AMC TFA Chemical Structure

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Based on 1 publication(s) in Google Scholar

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Description

D-Val-Leu-Lys-AMC TFA is a selective fluorogenic peptide substrate of plasmin. D-Val-Leu-Lys-AMC TFA can be used for the quantification of enzymatic activity of plasmin. Ex: 360-380 nm, Em: 440-460 nm[1].

Molecular Weight

743.69

Formula

C31H43F6N5O9

Appearance

Solid

Color

White to off-white

Sequence

{d-Val}-Leu-Lys-{AMC}

Sequence Shortening

{d-Val}-LK-{AMC}

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (134.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3446 mL 6.7232 mL 13.4465 mL
5 mM 0.2689 mL 1.3446 mL 2.6893 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3446 mL 6.7232 mL 13.4465 mL 33.6162 mL
5 mM 0.2689 mL 1.3446 mL 2.6893 mL 6.7232 mL
10 mM 0.1345 mL 0.6723 mL 1.3446 mL 3.3616 mL
15 mM 0.0896 mL 0.4482 mL 0.8964 mL 2.2411 mL
20 mM 0.0672 mL 0.3362 mL 0.6723 mL 1.6808 mL
25 mM 0.0538 mL 0.2689 mL 0.5379 mL 1.3446 mL
30 mM 0.0448 mL 0.2241 mL 0.4482 mL 1.1205 mL
40 mM 0.0336 mL 0.1681 mL 0.3362 mL 0.8404 mL
50 mM 0.0269 mL 0.1345 mL 0.2689 mL 0.6723 mL
60 mM 0.0224 mL 0.1121 mL 0.2241 mL 0.5603 mL
80 mM 0.0168 mL 0.0840 mL 0.1681 mL 0.4202 mL
100 mM 0.0134 mL 0.0672 mL 0.1345 mL 0.3362 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
D-Val-Leu-Lys-AMC TFA
Cat. No.:
HY-P4341A
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