Dansyl-Tyr-Val-Gly
Based on 1 Customer Validation
Dansyl-Tyr-Val-Gly is a substrate of peptidylglycine monooxygenase (PHM), with a KD of 22 μM for PHM. Dansyl-Tyr-Val-Gly can be used to investigate the catalytic mechanism, kinetics and inactivation characteristics of PHM.
For research use only. We do not sell to patients.
- Purity: 99.53%
- CAS No.: 113527-49-4
- Formula: C28H34N4O7S
- Molecular Weight:570.66
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Dansyl-Tyr-Val-Gly (15-25 μM) protects recombinant type A rat 75 kDa α-AE against ascorbate-mediated inactivation of its peptidyl-Gly hydroxylase activity and serves as a substrate for α-AE-mediated amidation to quantify residual enzyme activity[2].
Dansyl-Tyr-Val-Gly (15 μM) supports amidation with ascorbate as a reductant, which has an apparent Km of 0.20 mM and a relative Vm,app of 100% when incubated with recombinant type A rat 75 kDa α-AE[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 113527-49-4
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Appearance Solid
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Molecular Weight 570.66
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Formula C28H34N4O7S
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Color Off-white to light yellow
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Sequence Shortening
Dansyl-YVG
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (175.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Welch EF, et al. Pre-Steady-State Reactivity of Peptidylglycine Monooxygenase Implicates Ascorbate in Substrate Triggering of the Active Conformer. Biochemistry. 2022;61(8):665-677. [Content Brief]
[2]. Merkler DJ, et al. Selective inactivation of the hydroxylase activity of bifunctional rat peptidylglycine alpha-amidating enzyme. Arch Biochem Biophys. 1992;294(2):594-602. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7524 mL | 8.7618 mL | 17.5236 mL | 43.8089 mL |
| 5 mM | 0.3505 mL | 1.7524 mL | 3.5047 mL | 8.7618 mL | |
| 10 mM | 0.1752 mL | 0.8762 mL | 1.7524 mL | 4.3809 mL | |
| 15 mM | 0.1168 mL | 0.5841 mL | 1.1682 mL | 2.9206 mL | |
| 20 mM | 0.0876 mL | 0.4381 mL | 0.8762 mL | 2.1904 mL | |
| 25 mM | 0.0701 mL | 0.3505 mL | 0.7009 mL | 1.7524 mL | |
| 30 mM | 0.0584 mL | 0.2921 mL | 0.5841 mL | 1.4603 mL | |
| 40 mM | 0.0438 mL | 0.2190 mL | 0.4381 mL | 1.0952 mL | |
| 50 mM | 0.0350 mL | 0.1752 mL | 0.3505 mL | 0.8762 mL | |
| 60 mM | 0.0292 mL | 0.1460 mL | 0.2921 mL | 0.7301 mL | |
| 80 mM | 0.0219 mL | 0.1095 mL | 0.2190 mL | 0.5476 mL | |
| 100 mM | 0.0175 mL | 0.0876 mL | 0.1752 mL | 0.4381 mL |
Dansyl-Tyr-Val-Gly Related Classifications
HY-138126 Related Classifications
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- Dansyl-Tyr-Val-Gly
- 113527-49-4
- Others
- Cu(I)-CO species
- FTIR spectroscopy
- α-AE
- recombinant rat type A 75-kDa peptidylglycine α-amidating enzyme
- peptidylglycine monooxygenase
- ascorbate
- PHM
- peptidylglycine α-amidation pathway
- glycine residue’s α-carbon
- peptidyl-α-hydroxyglycine intermediate
- Inhibitor
- inhibitor
- inhibit