Brilliant Black BN
Based on 1 Customer Validation
Brilliant black BN (E151) is an azo dye and a food colorant. Brilliant black BN is a promising antiviral agent against EV71 infection via inhibiting the interaction between EV71 and its cellular uncoating factor cyclophilin A. Brilliant black BN has the potential for the investigation of contagious disease.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- CAS. Nr.: 2519-30-4
- Formel: C28H17N5Na4O14S4
- Molecular Weight:867.68
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Speicherung:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Biologische Aktivität
Beschreibung
IC50 & Target
IC50: 2.39 µM- 28.12 µM (various strains of EV71)[2]
In Vitro
Brilliant black BN inhibits the interaction between EV71 and its cellular uncoating factor cyclophilin A [1].Brilliant black BN inhibited the infection EV71 -GFP at a concentration of 300 μM as evidenced from the reduced GFP signals. Brilliant black BN exhibits the inhibition of EV71 -GFP as a dose-dependent manner in infected RD cells, exhibits an IC50 value of 10.1 μM[1].Brilliant black BN is able to inhibit all tested 28 EV71, CVA16 and CVA6 strains. In rhabdomyosarcoma cells, 50% inhibitory concentration 29 of the dye for various strains of EV71 ranged from 2.39 μM to 28.12 μM ,whereas its 50% cytotoxic concentration is 1870 μM [1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:14-days old AG129 mice with EV71[1]
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Dosage:200 mg/kg
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Administration:Intraperitoneal injection
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Result:Had a Protective eddect on EV71 challenged AG129 mice by dye E151.
Chemical Information
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CAS. Nr. 2519-30-4
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Appearance Solid
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Molecular Weight 867.68
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Formel C28H17N5Na4O14S4
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Color Brown to black
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SMILES
O=S(C1=C2C=C(S(=O)(O[Na])=O)C(/N=N/C3=C4C=C(S(=O)(O[Na])=O)C=CC4=C(/N=N/C5=CC=C(S(=O)(O[Na])=O)C=C5)C=C3)=C(O)C2=C(NC(C)=O)C=C1)(O[Na])=O
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Synonyms
E 151
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 125 mg/mL (144.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Protokoll
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
Reinheit & Dokumentation
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Data Sheet (278 KB)
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SDS (701 KB)
- English - EN (701 KB)
- Français - FR (701 KB)
- Deutsch - DE (701 KB)
- Norwegian - NO (701 KB)
- Español - ES (701 KB)
- Swedish - SV (701 KB)
- Italian - IT (701 KB)
- Korean - KR (701 KB)
- Portuguese - PT (701 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Lang W, et al. Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1(T) strain from the Mariana Trench.J Environ Manage. 2014 Jan;132:155-64. [Content Brief]
[2]. Meng T, et al. In Vitro and In Vivo Inhibition of the Infectivity of Human Enterovirus 71 by a Sulfonated Food Azo Dye, Brilliant Black BN.J Virol. 2019 Aug 13;93(17). [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1525 mL | 5.7625 mL | 11.5250 mL | 28.8125 mL |
| 5 mM | 0.2305 mL | 1.1525 mL | 2.3050 mL | 5.7625 mL | |
| 10 mM | 0.1152 mL | 0.5762 mL | 1.1525 mL | 2.8812 mL | |
| 15 mM | 0.0768 mL | 0.3842 mL | 0.7683 mL | 1.9208 mL | |
| 20 mM | 0.0576 mL | 0.2881 mL | 0.5762 mL | 1.4406 mL | |
| 25 mM | 0.0461 mL | 0.2305 mL | 0.4610 mL | 1.1525 mL | |
| 30 mM | 0.0384 mL | 0.1921 mL | 0.3842 mL | 0.9604 mL | |
| 40 mM | 0.0288 mL | 0.1441 mL | 0.2881 mL | 0.7203 mL | |
| 50 mM | 0.0230 mL | 0.1152 mL | 0.2305 mL | 0.5762 mL | |
| 60 mM | 0.0192 mL | 0.0960 mL | 0.1921 mL | 0.4802 mL | |
| 80 mM | 0.0144 mL | 0.0720 mL | 0.1441 mL | 0.3602 mL | |
| 100 mM | 0.0115 mL | 0.0576 mL | 0.1152 mL | 0.2881 mL |
Brilliant Black BN Related Classifications
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