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Art. -Nr.: HY-E70194

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Endoproteinase Glu-C (MS grade)

(Synonyms: V8 protease; Glu-C)

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Endoproteinase Glu-C (MS grade) is a serine proteinase. Endoproteinase GluC is able to hydrolyze some serpins and all classes of mammalian immunoglobulins.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

CAS. Nr.: 137010-42-5

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Endoproteinase Glu-C (MS grade) Chemical Structure

CAS. Nr.: 137010-42-5

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Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Endoproteinase Glu-C (MS grade) is a serine proteinase. Endoproteinase GluC is able to hydrolyze some serpins and all classes of mammalian immunoglobulins^[1].

In Vitro

This product can be used for: protein enzyme digestion sequencing analysis, peptide map analysis and peptide mass fingerprint analysis.
Reaction conditions
Reaction pH: active between 4.0-10.0
Optimum pH: 8.0-8.5
Inhibitors: Diisopropylfluorophosphate (DFP), α2-macroglobulin, and Nα-P-tosyl-L-lysine chloromethyl ketone (TLCK)
Molecular weight: 24.0±2.4 kDa (SDS-PAGE)
Protocol
1）Dissolution: 25-50 mM NH4HCO3 (pH 7.8) to dissolve the target protein. If the solubility is not good, the target protein can be denatured (adding urea, SDS, DTT or heating).
2）Enzyme digestion: The recommended ratio of V8 protease and target protein is 1/20-1/100 (W/W), enzyme digestion at 25°C or 37°C for 2-18 h.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 137010-42-5
Appearance Solid
Color White to off-white
SMILES

[Endoproteinase Glu-C (MS grade)]
Synonyms

V8 protease; Glu-C
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (264 KB)
SDS (704 KB)
- English - EN (704 KB)
- Français - FR (704 KB)
- Deutsch - DE (704 KB)
- Norwegian - NO (704 KB)
- Español - ES (704 KB)
- Swedish - SV (704 KB)
- Italian - IT (704 KB)
- Korean - KR (704 KB)
- Portuguese - PT (704 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Burchacka E, et, al. Phosphonic analogues of glutamic acid as irreversible inhibitors of Staphylococcus aureus endoproteinase GluC: an efficient synthesis and inhibition of the human IgG degradation. Bioorg Med Chem Lett. 2013 Mar 1;23(5):1412-5. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Endoproteinase Glu-C (MS grade)

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Endoproteinase Glu-C (MS grade)

Biologische Aktivität

Chemical Information

Reinheit & Dokumentation

Verweise

Calculators

Help & FAQs

Endoproteinase Glu-C (MS grade) Related Classifications

HY-E70194 Related Classifications