RUSKI-201 dihydrochloride
Based on 1 Customer Validation
RUSKI-201 dihydrochloride is a potent and specific Hedgehog acyltransferase (Hhat) inhibitor with an IC50 of 0.20 μM. RUSKI-201 dihydrochloride is able to block Hh signaling from Shh overexpressing cells and inhibits Hh palmitoylation. RUSKI-201 dihydrochloride is potential Hhat chemical probe in cells and can used in studies of Hhat catalytic function.
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- Reinheit : 98.2%
- CAS. Nr.: 2320262-09-5
- Formel: C20H29Cl2N3OS
- Molecular Weight:430.43
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Speicherung:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biologische Aktivität
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Chemical Information
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Biologische Aktivität
Beschreibung
In Vitro
RUSKI-201 (0.5-25 μM; 48 hours) has no effect on cell viability at concentrations >25 μM in Shh-Light2 cells derived from NIH3T3 cells stably transfected with a Gli-responsive firefly luciferase HEK-293 cells stably overexpressing Shh[1]. RUSKI-201 inhibits signaling in H520, Panc-1, and MCF-7 coculture with Shh-Light2 cells (IC50=4.8±0.60 μM, 7.8±1.3 μM, and 8.5±0.65 μM, respectively)[1]RUSKI-201 (0.01-10 μM; 24 hours) induces a selective inhibition of Shh palmitoylation and does not affect global palmitoylation levels in HEK-293 Shh+ cells treated with RUSKI-201 followed by YnPal and functionalized with AzTB[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HEK-293 Shh+ cells
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Concentration:0.01 μM, 0.05 μM, 0.1 μM, 0.5 μM, 1 μM, 5 μM, 10 μM
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Incubation Time:24 hours
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Result:Decreased YnPal-Shh expression without α-Shh change; Capable of blocking Hh signaling from Shh overexpressing cells.
Chemical Information
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CAS. Nr. 2320262-09-5
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Appearance Solid
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Molecular Weight 430.43
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Formel C20H29Cl2N3OS
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Color Light brown to brown
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SMILES
CCC(CNCC(N1CCC2=C(C1C3=NC(C)=CC=C3)C=CS2)=O)C.Cl.Cl
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Protokoll
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Reinheit & Dokumentation
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Data Sheet (270 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
RUSKI-201 dihydrochloride Related Classifications
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