MCLA hydrochloride
Based on 2 publication(s) in Google Scholar
MCLA hydrochloride is a chemiluminescent reagent which can be used to quantify aqueous concentrations of superoxide.
For research use only. We do not sell to patients.
- Purity: 99.09%
- CAS No.: 128322-44-1
- Formula: C14H14ClN3O2
- Molecular Weight:291.73
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) MCLA hydrochloride
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Biological Activity
The non-specific luminescence remains almost constant for 10 min after the addition of MCLA hydrochloride (MCLA) and is not significantly influenced by SOD. The MCLA hydrochloride method is, however, 4.5-times more sensitive than the CLA method[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 128322-44-1
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Appearance Solid
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Molecular Weight 291.73
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Formula C14H14ClN3O2
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Color Light yellow to yellow
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SMILES
CC1=NC2=CNC(C3=CC=C(OC)C=C3)=CN2C1=O.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Redox Biol
A closer look into NADPH oxidase inhibitors: Validation and insight into their mechanism of action. [Abstract]2020 May;32:101466. PMID: 32105983 -
Talanta
Can we determine superoxide ion reliably? - Automatic flow system for the measurement of the superoxide lifetime in varied aqueous media. [Abstract]2026 Oct 1:308:129826. PMID: 42008912
Solvent & Solubility
DMSO : 10 mg/mL (34.28 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
The standard reaction mixture contains 1×l0-7 M MCLA hydrochloride (MCLA), 5×10-5 M hypoxanthine, XOD (6.5 U), SOD (0.2 to 20 ng/mL) or none, and 50 mM Tris-HCl buffer containing 0.1 mM EDTA at pH 7.8, in a total volume of 3.0 mL. Chemiluminescence measurement is initiated by the addition of MCLA hydrochloride to the standard incubation mixture excluding XOD, continued for 4 min without additive and for an additional 4 min after the addition of XOD. Chemiluminescence is measured using a luminescence reader at 25°C[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4278 mL | 17.1391 mL | 34.2783 mL | 85.6957 mL |
| 5 mM | 0.6856 mL | 3.4278 mL | 6.8557 mL | 17.1391 mL | |
| 10 mM | 0.3428 mL | 1.7139 mL | 3.4278 mL | 8.5696 mL | |
| 15 mM | 0.2285 mL | 1.1426 mL | 2.2852 mL | 5.7130 mL | |
| 20 mM | 0.1714 mL | 0.8570 mL | 1.7139 mL | 4.2848 mL | |
| 25 mM | 0.1371 mL | 0.6856 mL | 1.3711 mL | 3.4278 mL | |
| 30 mM | 0.1143 mL | 0.5713 mL | 1.1426 mL | 2.8565 mL |
MCLA hydrochloride Related Classifications
HY-W013275 Related Classifications
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