Highly sensitive and reliable chemiluminescence method for the assay of superoxide dismutase in human erythrocytes

  • FEBS Lett. 1988 Nov 7;239(2):347-50. doi: 10.1016/0014-5793(88)80949-9.
H Kimura  1 ,  M Nakano
Affiliations
  • 1. College of Medical Care and Technology, Gunma University, Japan.
PMID: 2846362 DOI: 10.1016/0014-5793(88)80949-9
Abstract

Superoxide dismutase in human erythrocytes was assayed by the inhibition of highly diluted erythrocyte lysates on 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazol[1,2-alpha]pyra zin-3-one- dependent luminescence induced by the hypoxanthine-xanthine oxidase system. Our chemiluminescence procedure gave 95-times higher sensitivity than the cytochrome c method. The concentration of superoxide dismutase in erythrocytes of Down's syndrome patients was approx. 1.8-times higher than that of normal humans.

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