1. Dye Reagents

MCLA hydrochloride 

Cat. No.: HY-W013275
Handling Instructions

MCLA hydrochloride is a chemiluminescent reagent which can be used to quantify aqueous concentrations of superoxide.

For research use only. We do not sell to patients.

MCLA hydrochloride Chemical Structure

MCLA hydrochloride Chemical Structure

CAS No. : 128322-44-1

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

MCLA hydrochloride is a chemiluminescent reagent which can be used to quantify aqueous concentrations of superoxide.

In Vitro

The non-specific luminescence remains almost constant for 10 min after the addition of MCLA hydrochloride (MCLA) and is not significantly influenced by SOD. The MCLA hydrochloride method is, however, 4.5-times more sensitive than the CLA method[1].

References
Kinase Assay
[1]

The standard reaction mixture contains 1×l0-7 M MCLA hydrochloride (MCLA), 5×10-5 M hypoxanthine, XOD (6.5 U), SOD (0.2 to 20 ng/mL) or none, and 50 mM Tris-HCl buffer containing 0.1 mM EDTA at pH 7.8, in a total volume of 3.0 mL. Chemiluminescence measurement is initiated by the addition of MCLA hydrochloride to the standard incubation mixture excluding XOD, continued for 4 min without additive and for an additional 4 min after the addition of XOD. Chemiluminescence is measured using a luminescence reader at 25°C[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

291.73

Formula

C₁₄H₁₄ClN₃O₂

CAS No.

128322-44-1

SMILES

CC1=NC2=CNC(C3=CC=C(OC)C=C3)=CN2C1=O.Cl

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
MCLA hydrochloride
Cat. No.:
HY-W013275
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MCLA hydrochloride

Cat. No.: HY-W013275