Oxonol VI
Based on 1 Customer Validation
Oxonol VI is an optical indicator of membrane potential in lipid vesicles (excitation/emission wavelengths: 614/646 nm). Oxonol VI can be used to detect changes in membrane potential associated with (Na+ + K+)-ATPase activity in reconstituted vesicles.
For research use only. We do not sell to patients.
- Purity: 98.18%
- CAS No.: 64724-75-0
- Formula: C17H20N2O4
- Molecular Weight:316.35
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (The following is our recommended protocol. This protocol is for guidance only and should be modified according to your specific needs).
1 Preparation of dye stock solution
1) Prepare a 3.16 mM Oxonol VI stock solution in ethanol.
2 Preparation of dye working solution
1) Dilute the 3.16 mM Oxonol VI stock solution with a mixture of ethanol and water at a volume ratio of 1:5 (ethanol:water).
2) Adjust the dilution ratio to ensure that the final concentration of Oxonol VI reaches the range of 10-500 nM.
3. Specific steps
1) Add 1 mL of the appropriate buffer to the fluorescence cuvette and equilibrate to the set temperature (20 °C by default unless otherwise specified).
2) Measure the background fluorescence intensity of the buffer in the cuvette.
3) Add 5 μL of the prepared Oxonol VI working solution to the cuvette containing the equilibrated buffer.
4) After the fluorescence signal stabilizes, add a predetermined amount of vesicle suspension to the cuvette.
5) Continuously monitor the fluorescence signal using a spectrophotometer. Calculate the relative fluorescence change caused by the addition of the vesicle suspension, using the baseline fluorescence intensity recorded before the addition of the vesicles as a reference.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
630
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Chemical Information
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CAS No. 64724-75-0
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Appearance Solid
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Molecular Weight 316.35
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Formula C17H20N2O4
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Color Brown to black
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SMILES
O=C1ON=C(CCC)/C1=C\C=C\C=C\C2=C(ON=C2CCC)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (316.11 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. H J Apell, et al. Oxonol VI as an optical indicator for membrane potentials in lipid vesicles. Biochim Biophys Acta. 1987 Oct 16;903(3):480-94. [Content Brief]
[2]. Pouliquin P, et al. Electrophysiological study with oxonol VI of passive NO3- transport by isolated plant root plasma membrane. Biophys J. 1999 Jan;76(1 Pt 1):360-73. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1611 mL | 15.8053 mL | 31.6106 mL | 79.0264 mL |
| 5 mM | 0.6322 mL | 3.1611 mL | 6.3221 mL | 15.8053 mL | |
| 10 mM | 0.3161 mL | 1.5805 mL | 3.1611 mL | 7.9026 mL | |
| 15 mM | 0.2107 mL | 1.0537 mL | 2.1074 mL | 5.2684 mL | |
| 20 mM | 0.1581 mL | 0.7903 mL | 1.5805 mL | 3.9513 mL | |
| 25 mM | 0.1264 mL | 0.6322 mL | 1.2644 mL | 3.1611 mL | |
| 30 mM | 0.1054 mL | 0.5268 mL | 1.0537 mL | 2.6342 mL | |
| 40 mM | 0.0790 mL | 0.3951 mL | 0.7903 mL | 1.9757 mL | |
| 50 mM | 0.0632 mL | 0.3161 mL | 0.6322 mL | 1.5805 mL | |
| 60 mM | 0.0527 mL | 0.2634 mL | 0.5268 mL | 1.3171 mL | |
| 80 mM | 0.0395 mL | 0.1976 mL | 0.3951 mL | 0.9878 mL | |
| 100 mM | 0.0316 mL | 0.1581 mL | 0.3161 mL | 0.7903 mL |
Oxonol VI Related Classifications
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