Dexamethasone hemisuccinate
Based on 1 Customer Validation
Dexamethasone hemisuccinate is a glucocorticoid prodrug and an inhibitor of IL-6 release, and its liposomal formulation is internalized by retinal pigment epithelial cells. Dexamethasone hemisuccinate binds to retinal pigment epithelial cells in a time-dependent manner and exhibits no cytotoxicity toward these cells. Dexamethasone hemisuccinate can be used in research on age-related macular degeneration.
For research use only. We do not sell to patients.
- CAS No.: 3800-86-0
- Formula: C26H33FO8
- Molecular Weight:492.53
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
IL-6 |
In Vitro
Dexamethasone hemisuccinate-loaded liposomes (0.1-1.0 M calcium acetate; 20-120 min) are prepared by a microfluidic method, which exhibits higher loading capacity compared with the thin-film hydration method[1].
Dexamethasone hemisuccinate-loaded liposomes (10 mg/mL; 30 days) exhibit a biphasic release profile, with an initial burst followed by sustained release over approximately four weeks, where microfluidic formulations show a higher burst release[1].
Dexamethasone hemisuccinate (1-10 mM) modulates IL-6 release in LPS-activated ARPE-19 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:ARPE-19 cells
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Concentration:1 and 10 µM dexamethasone hemisuccinate loaded liposomes
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Incubation Time:24 h
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Result:Significantly reduced the pro-inflammatory activity induced by LPS.
At 1 µM dexamethasone concentration, the liposomes obtained by thin-film hydration and microfluidics were 2.3-times and 3-times more efficient than free dexamethasone, respectively.
Empty liposomes produced with both techniques did not present anti-inflammatory activity.
Control plain liposomes did not display pro-inflammatory activity on non-stimulated cells.
Chemical Information
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CAS No. 3800-86-0
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Appearance Solid
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Molecular Weight 492.53
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Formula C26H33FO8
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SMILES
O=C1C=C[C@]2(C(CC[C@@]3([C@]2([C@H](C[C@]4([C@]3(C[C@@H]([C@@]4(C(COC(CCC(O)=O)=O)=O)O)C)[H])C)O)F)[H])=C1)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (203.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Research Protocol for Endocrine Diseases
Endocrine diseases often arise from disrupted hormone production, hormone signaling, or target-tissue responsiveness; for diabetes-focused endocrine disease models, insulin signaling regulates glucose uptake, hepatic glucose output, lipid metabolism, and β-cell compensation. Type 2 diabetes develops through interacting defects in insulin resistance, β-cell dysfunction, adipose inflammation, hepatic glucose overproduction, altered incretin signaling, and ectopic lipid metabolism. A major unresolved question is whether endocrine dysfunction is driven primarily by target-tissue insulin resistance, intrinsic β-cell failure, immune/inflammatory stress, or combined multi-organ failure that differs by disease stage.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.0303 mL | 10.1517 mL | 20.3033 mL | 50.7583 mL |
| 5 mM | 0.4061 mL | 2.0303 mL | 4.0607 mL | 10.1517 mL | |
| 10 mM | 0.2030 mL | 1.0152 mL | 2.0303 mL | 5.0758 mL | |
| 15 mM | 0.1354 mL | 0.6768 mL | 1.3536 mL | 3.3839 mL | |
| 20 mM | 0.1015 mL | 0.5076 mL | 1.0152 mL | 2.5379 mL | |
| 25 mM | 0.0812 mL | 0.4061 mL | 0.8121 mL | 2.0303 mL | |
| 30 mM | 0.0677 mL | 0.3384 mL | 0.6768 mL | 1.6919 mL | |
| 40 mM | 0.0508 mL | 0.2538 mL | 0.5076 mL | 1.2690 mL | |
| 50 mM | 0.0406 mL | 0.2030 mL | 0.4061 mL | 1.0152 mL | |
| 60 mM | 0.0338 mL | 0.1692 mL | 0.3384 mL | 0.8460 mL | |
| 80 mM | 0.0254 mL | 0.1269 mL | 0.2538 mL | 0.6345 mL | |
| 100 mM | 0.0203 mL | 0.1015 mL | 0.2030 mL | 0.5076 mL |
Dexamethasone hemisuccinate Related Classifications
HY-W745334 Related Classifications
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Keywords
- Dexamethasone hemisuccinate
- 3800-86-0
- Interleukin Related
- microfluidic procedure
- age-related macular degeneration
- glucocorticoid prodrug
- IL-6 release inhibitor
- LPS-stimulated ARPE-19 cells
- ARPE-19 cells
- LPS-activated ARPE-19 cells
- thin-film hydration method
- retinal pigment epithelial cells
- liposomal formulations
- Inhibitor
- inhibitor
- inhibit