Diosmetin-7-O-β-D-glucopyranoside
Based on 1 Customer Validation
Diosmetin-7-O-β-D-glucopyranoside is a natural product that can be isolated from the flowers of Chrysanthemum morifolium. Diosmetin-7-O-β-D-glucopyranoside has potent antiviral and anti-inflammatory activities against SARS-CoV-2 both in vitro (IC50 = 0.74 μM) and in vivo. Diosmetin-7-O-β-D-glucopyranoside has significant anti-thrombotic activity when combined with Pae and 5-HMF at a ratio of 3:4:3 in a zebrafish model.
For research use only. We do not sell to patients.
- Purity : 99.61%
- CAS No.: 20126-59-4
- Formula: C22H22O11
- Molecular Weight:462.40
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| RAW264.7 | IC50 |
>50 μM
Compound: 6
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Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as decrease in PGE2 production preincubated for 1 hr followed by LPS stimulation and measured after 24 hrs by ELISA
Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as decrease in PGE2 production preincubated for 1 hr followed by LPS stimulation and measured after 24 hrs by ELISA
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[PMID: 31747281] |
In Vitro
Diosmetin-7-O-β-D-glucopyranoside (Compound DG) (0-300 μM, 48 h) has strong antiviral activity against SARS-CoV-2 in BEAS-2B cells (IC50 = 0.74 μM)[3].
Diosmetin-7-O-β-D-glucopyranoside (12.5-100 μM) shows low cytotoxicity in mouse macrophage hACE2-expressing RAW264.7 cells[3].
Diosmetin-7-O-β-D-glucopyranoside (0.625-2.5 μM) decreases the apoptotic rates of SARS-CoV-2-infected cells as well as viral loads in a concentration-dependent manner[3].
Diosmetin-7-O-β-D-glucopyranoside (0.625-10 μM) sharply reduces the expression in wild-type YTHDF1-transfected H293T cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:BEAS-2B cells
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Concentration:0, 50, 100, 150, 200, 250, 300 μM
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Incubation Time:At different stages of viral infection: post-infection treatment, cell pretreatment, and virus pretreatment
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Result:Presented stronger inhibition on viral replication than viral penetration.
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Cell Line:SARS-CoV-2-infected RAW246.7 cells
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Concentration:0.625, 1.25, 2.5 μM
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Incubation Time:24 h
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Result:Reduced the expression of viral NP, HK3, NF-κB p-p65 and IL-6 in a concentration-dependent manner.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:60 male H11-K18-hACE2 transgenic mice[3]
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Dosage:25, 50, 100 mg/kg
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Administration:Intraperitoneal injection (i.p.) for 5 days
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Result:Improved SARS-CoV-2-induced inflammatory cell infiltration.
Reduced the pathological scores and viral loads.
Declined the levels of inflammatory cytokines in a dose-dependent manner.
Effectively reduced the neutrophil infiltration, promoted M1 macrophage to M2 polarization, and altered the expression of M1/M2 genetic markers.
Chemical Information
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CAS No. 20126-59-4
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Appearance Solid
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Molecular Weight 462.40
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Formula C22H22O11
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Color White to yellow
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SMILES
O=C(C=C(C1=CC(O)=C(OC)C=C1)OC2=CC(O[C@@H]([C@@H]([C@@H](O)[C@@H]3O)O)O[C@@H]3CO)=C4)C2=C4O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (108.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.41 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.41 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Wang YJ, et al. Studies on chemical constituents in Huangjuhua (flowers of Chrysanthemum morifolium. Zhongguo Zhong Yao Za Zhi. 2008 Mar;33(5):526-30. [Content Brief]
[3]. Xu, Y. L., et al., (2025). Diosmetin-7-O-β-D-glucopyranoside from Pogostemonis Herba alleviated SARS-CoV-2-induced pneumonia by reshaping macrophage polarization and limiting viral replication. Journal of ethnopharmacology, 336, 118704. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1626 mL | 10.8131 mL | 21.6263 mL | 54.0657 mL |
| 5 mM | 0.4325 mL | 2.1626 mL | 4.3253 mL | 10.8131 mL | |
| 10 mM | 0.2163 mL | 1.0813 mL | 2.1626 mL | 5.4066 mL | |
| 15 mM | 0.1442 mL | 0.7209 mL | 1.4418 mL | 3.6044 mL | |
| 20 mM | 0.1081 mL | 0.5407 mL | 1.0813 mL | 2.7033 mL | |
| 25 mM | 0.0865 mL | 0.4325 mL | 0.8651 mL | 2.1626 mL | |
| 30 mM | 0.0721 mL | 0.3604 mL | 0.7209 mL | 1.8022 mL | |
| 40 mM | 0.0541 mL | 0.2703 mL | 0.5407 mL | 1.3516 mL | |
| 50 mM | 0.0433 mL | 0.2163 mL | 0.4325 mL | 1.0813 mL | |
| 60 mM | 0.0360 mL | 0.1802 mL | 0.3604 mL | 0.9011 mL | |
| 80 mM | 0.0270 mL | 0.1352 mL | 0.2703 mL | 0.6758 mL | |
| 100 mM | 0.0216 mL | 0.1081 mL | 0.2163 mL | 0.5407 mL |
Diosmetin-7-O-β-D-glucopyranoside Related Classifications
HY-N0713 Related Classifications
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