DSG-PEG5000-Alkyne
DSG-PEG5000-Alkyne is a conjugate composed of DSG, a PEG chain, and a terminal alkyne group. DSG-PEG5000-Alkyne combines the membrane compatibility of phospholipids with the high reactivity of the alkyne group in click chemistry, making it suitable for research in biomaterial construction and drug delivery.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 分子量:5000 (Average)
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
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Help & FAQs
生物活性
製品説明
体外実験
The structure of DSG-PEG-Alkyne comprises three key components that work synergistically:
DSG: Acts as a hydrophobic anchor, securely embedding the entire molecule into the membrane structure of lipid nanoparticles.
PEG chain: Acts as a hydrophilic spacer, forming a hydration layer on the particle surface, reducing nonspecific adsorption and immune system clearance, thus prolonging the carrier's circulation time in vivo.
Alkyne group: Can undergo click chemistry reactions, achieving specific linkage with molecules containing an azide group.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
化学情報
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分子量 5000 (Average)
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SMILES
CCCCCCCCCCCCCCCCCC(OCC(COCCOCC#C)OC(CCCCCCCCCCCCCCCCC)=O)=O.[n]
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
純度とドキュメンテーション
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
DSG-PEG5000-Alkyne 関連分類
HY-183172C 関連分類
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