DSPE-PEG2000-Azide ammonium

DSPE-PEG2000-Azide ammonium-containing liposomes successfully conjugate with DBCO-modified antibodies and SOD via strain-promoted alkyne-azide cycloaddition, producing purified immunoliposomes with ~50 antibodies per liposome and no detectable size change^[1].
DSPE-PEG2000-Azide ammonium stably inserts into the lipid bilayer of bovine milk exosomes via hydrophobic interaction, incorporating ~394 PEG chains per exosome at a 20000:1 molar ratio, modifying surface charge without altering particle size, and remaining stable in PBS and mucin environments^[2].
DSPE-PEG2000-Azide ammonium modified bovine milk exosomes exhibit significantly enhanced stability in acidic buffers mimicking gastric conditions, with half the protein loss of unmodified exosomes at pH 2.2 and pH 4.5^[2].
DSPE-PEG2000-Azide ammonium modified bovine milk exosomes exhibit 3.2-fold enhanced permeability across an in vitro intestinal mucus layer compared to unmodified exosomes^[2].
DSPE-PEG2000-Azide ammonium modified bovine milk exosomes loaded with GFP-targeting siRNA mediate 60% silencing of GFP expression in HEK293 cells, with only slightly reduced efficacy compared to unmodified siRNA-loaded exosomes^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.