Eicosapentaenoic acid methyl ester
Based on 1 Customer Validation
Eicosapentaenoic acid methyl ester is a degradant of a monogalactosyl diacylglycerol (an anticancer compound by inducing apoptosis).
For research use only. We do not sell to patients.
- Purity: 99.11%
- CAS No.: 2734-47-6
- Formula: C21H32O2
- Molecular Weight:316.49
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Storage:Pure form -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
Biological Activity
Chemical Information
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CAS No. 2734-47-6
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Appearance Liquid (Density: 0.912±0.06 g/cm3)
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Molecular Weight 316.49
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Formula C21H32O2
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Color Colorless to light yellow
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SMILES
CC/C=C\C/C=C\C/C=C\C/C=C\C/C=C\CCCC(OC)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (315.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.90 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (913 KB)
- English - EN (913 KB)
- Français - FR (913 KB)
- Deutsch - DE (913 KB)
- Norwegian - NO (913 KB)
- Español - ES (913 KB)
- Swedish - SV (913 KB)
- Italian - IT (913 KB)
- Korean - KR (913 KB)
- Portuguese - PT (913 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1597 mL | 15.7983 mL | 31.5966 mL | 78.9914 mL |
| 5 mM | 0.6319 mL | 3.1597 mL | 6.3193 mL | 15.7983 mL | |
| 10 mM | 0.3160 mL | 1.5798 mL | 3.1597 mL | 7.8991 mL | |
| 15 mM | 0.2106 mL | 1.0532 mL | 2.1064 mL | 5.2661 mL | |
| 20 mM | 0.1580 mL | 0.7899 mL | 1.5798 mL | 3.9496 mL | |
| 25 mM | 0.1264 mL | 0.6319 mL | 1.2639 mL | 3.1597 mL | |
| 30 mM | 0.1053 mL | 0.5266 mL | 1.0532 mL | 2.6330 mL | |
| 40 mM | 0.0790 mL | 0.3950 mL | 0.7899 mL | 1.9748 mL | |
| 50 mM | 0.0632 mL | 0.3160 mL | 0.6319 mL | 1.5798 mL | |
| 60 mM | 0.0527 mL | 0.2633 mL | 0.5266 mL | 1.3165 mL | |
| 80 mM | 0.0395 mL | 0.1975 mL | 0.3950 mL | 0.9874 mL | |
| 100 mM | 0.0316 mL | 0.1580 mL | 0.3160 mL | 0.7899 mL |