eIF4A-IN-3
Based on 1 Customer Validation
eIF4A-IN-3, Silvestrol (HY-13251) analogue, is an eIF4A inhibitor. eIF4A-IN-3 blocks protein translation initiation by interfering with eIF4F complex assembly, preferentially inhibiting translation of mRNAs with long, highly structured 5'-untranslated regions. eIF4A-IN-3 inhibits proliferation of human breast cancer cells. eIF4A-IN-3 has moderate apical to basolateral permeability in intestinal cell monolayers and a low efflux ratio.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 1402931-85-4
- Formula: C28H28ClNO6
- Molecular Weight:509.98
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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eIF4A |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MDA-MB-231 | EC50 |
0.4 nM
Compound: 75
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Growth inhibition of human MDA-MB-231 cells incubated for 72 hrs by MTS assay
Growth inhibition of human MDA-MB-231 cells incubated for 72 hrs by MTS assay
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[PMID: 23025805] |
| MDA-MB-231 | EC50 |
0.6 nM
Compound: 75
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Inhibition of eIF4FA in human MDA-MB-231 cells transfected with c-myc- 5'-UTR-luciferase construct assessed as reduction in translation initiation incubated for 24 hrs by differential translation assay
Inhibition of eIF4FA in human MDA-MB-231 cells transfected with c-myc- 5'-UTR-luciferase construct assessed as reduction in translation initiation incubated for 24 hrs by differential translation assay
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[PMID: 23025805] |
| MDA-MB-231 | EC50 |
15 nM
Compound: 75
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Inhibition of eIF4FA in human MDA-MB-231 cells transfected with tubulin 5'-UTR-luciferase construct assessed as reduction in translation initiation incubated for 24 hrs by differential translation assay
Inhibition of eIF4FA in human MDA-MB-231 cells transfected with tubulin 5'-UTR-luciferase construct assessed as reduction in translation initiation incubated for 24 hrs by differential translation assay
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[PMID: 23025805] |
eIF4A-IN-3 (Compound 75) (24 h) potently inhibits translation initiation of mRNAs with highly structured 5′UTRs in MDA-MB-231 human breast cancer cells, with a myc-LUC EC50 of 0.6 nM and tub-LUC EC50 of 15 nM[1].
eIF4A-IN-3 (72 h) potently inhibits the proliferation of MDA-MB-231 human breast cancer cells, with a growth inhibition EC50 of 0.4 nM[1].
eIF4A-IN-3 has moderate permeability across Caco-2 cell monolayers (15.5 × 10-6 cm/s) and a low efflux ratio of 2.0, indicating minimal interaction with P-glycoprotein[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1402931-85-4
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Appearance Solid
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Molecular Weight 509.98
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Formula C28H28ClNO6
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Color White to off-white
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SMILES
COC1=CC=C([C@@]23OC4=CC(Cl)=CC(OC)=C4[C@]2(O)[C@@H]([C@@H]([C@H]3C5=CC=CC=C5)C(N(C)C)=O)O)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Purity & Documentation
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Data Sheet (281 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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