Energy-coupling factor-IN-1
Energy-coupling factor-IN-1 is an energy-coupling factor (ECF) transporter inhibitor with an IC50 of 42.84 μM against ECF-FolT2. Energy-coupling factor-IN-1 binds to the interface between the S component and the ECF module, and inhibits the uptake of folic acid and pantothenic acid via the target transporter. Energy-coupling factor-IN-1 acts as a bactericidal agent, exerting rapid and sustained activity for 24 h against Enterococcus faecium, while also acting against Gram-positive bacteria including drug-resistant strains. Energy-coupling factor-IN-1 improves the survival rate of zebrafish embryos infected with Enterococcus faecium. Energy-coupling factor-IN-1 can be used in studies of Gram-positive bacterial infections.
For research use only. We do not sell to patients.
- CAS No.: 3085883-97-9
- Formula: C28H29ClN4O5
- Molecular Weight:537.01
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Energy-coupling factor-IN-1 (compound 36) potently inhibits ECF transporter-mediated substrate uptake in Lactobacillus casei whole cells with an IC50 of 7 μM[1].
Energy-coupling factor-IN-1 inhibits both ECF-FolT2 and ECF-PanT transporters in Lactobacillus delbrueckii proteoliposomes, with an IC50 of 42.84 μM for ECF-FolT2 and near-complete inhibition of ECF-PanT at 150 μM[1].
Energy-coupling factor-IN-1 exhibits potent antibacterial activity against a panel of Gram-positive bacteria, including vancomycin-resistant E. faecium and penicillin-resistant S. pneumoniae, with MIC values ranging from 0.5-4 μg/mL[1].
Energy-coupling factor-IN-1 (17.2-68.8 μg/mL; 24 h) displays rapid, sustained bactericidal activity against multidrug-resistant Enterococcus faecium ATCC51559[1].
Energy-coupling factor-IN-1 binds to the pantothenate-binding pocket of Streptococcus pneumoniae ECF-PanT, forming interactions with both the S-component and EcfT module residues to enhance binding affinity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 3085883-97-9
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Molecular Weight 537.01
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Formula C28H29ClN4O5
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SMILES
O=C(O)C1=CC(OCC2=CC(C3=CC(Cl)=C(OC(C)C)N=C3)=CC(N4N=NC(C(C)(C)C)=C4)=C2)=CC=C1O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Energy-coupling factor-IN-1 Related Classifications
HY-184417 Related Classifications
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