Ferrozine sodium hydrate

Cat. No.: HY-W151629A Purity: 99.80%: Data Sheet
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Ferrozine sodium hydrate is a spectrophotometric reagent for iron, can react with divalent Fe to form a stable magenta complex species. The complex has an absorption peak at 562 nm.Ferrozine sodium hydrate-based colorimetric assays can quantify iron in cells

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Ferrozine sodium hydrate Chemical Structure

CAS No. : 1266615-85-3

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Description

Ferrozine sodium hydrate is a spectrophotometric reagent for iron, can react with divalent Fe to form a stable magenta complex species. The complex has an absorption peak at 562 nm^[1]^[2].Ferrozine sodium hydrate-based colorimetric assays can quantify iron in cells^[3]

Formula

C₂₀H₁₄N₄O₆S₂.xH2O.Na

CAS No.

1266615-85-3

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=S(O)(C1=CC=C(C2=C(C3=CC=C(S(=O)(O[Na])=O)C=C3)N=C(C4=CC=CC=N4)N=N2)C=C1)=O.O.[x]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 11.67 mg/mL (ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.80%

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Data Sheet (274 KB) SDS (252 KB)

COA (258 KB) HNMR (295 KB) LCMS (108 KB)

Handling Instructions (2659 KB)

References

[1]. Ferrozine-A New Spectrophotometric Reagent for Iron. ANALYTICAL CHEMISTRY, VOL. 42, NO. 7, JUNE 1970. 779-781

[2]. E Viollier, et al. The ferrozine method revisited: Fe(II)/Fe(III) determination in natural waters.

[3]. Riemer et al.Colorimetric ferrozine-based assay for the quantitation of iron in cultured cells. Anal Biochem. 2004 Aug 15;331(2):370-5. [Content Brief]