FITC-dextran sulfate (MW 4kDa)
Based on 1 Customer Validation
FITC-dextran sulfate (MW 4kDa) is a dextran sulfate labeled with FITC (HY-66019), with an average molecular weight of 4 kDa. FITC-dextran sulfate (MW 4kDa) serves as an anionic macromolecular tracer. FITC-dextran sulfate (MW 4kDa) can be used for permeability studies.
For research use only. We do not sell to patients.
- Molecular Weight:4000.00 (Approximately)
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
FITC-dextran sulfate (MW 4kDa) exhibits significantly lower permeability across confluent BALB/c mouse brain microvascular endothelial cell monolayers than neutral 4kDa FITC-dextran, with greater permeability than 10kDa FITC-dextran sulfate[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Appearance Solid
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Molecular Weight 4000.00 (Approximately)
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Color Light yellow to yellow
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SMILES
[FITC-dextran sulfate]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Protocols
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Transepithelial/transendothelial electrical resistance assay
TEER measures electrical resistance across epithelial or endothelial monolayers cultured on permeable supports, and the readout reflects ionic conductance through the cell barrier, especially the paracellular pathway regulated by junctional integrity. TEER can be measured without destroying the monolayer and is commonly used before or during transport, permeability, barrier-disruption, and barrier-maturation experiments. TEER values are influenced by biological maturation and technical conditions; reported factors include temperature, medium formulation, passage number, electrode geometry, membrane properties, and junctional length during early monolayer maturation. Therefore, TEER should be interpreted with blank-insert subtraction, area normalization, repeated readings, and, when possible, orthogonal barrier readouts such as FITC-dextran flux or tight-junction staining.
Purity & Documentation
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Data Sheet (267 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
FITC-dextran sulfate (MW 4kDa) Related Classifications
HY-172163 Related Classifications
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Keywords
- FITC-dextran sulfate (MW 4kDa)
- Fluorescent Dye
- BALB/c mouse brain microvascular endothelial cell monolayers
- blood-brain barrier models
- endothelial cell surface glycocalyx
- mouse brain microvascular endothelial cell monolayers
- permeability inhibitor
- restricted permeability substrate
- anionic macromolecular tracer
- Inhibitor
- inhibitor
- inhibit