fMIFL
Based on 1 Customer Validation
fMIFL is a formyl tetrapeptide derived from Staphylococcus aureus, and also an agonist of mouse formyl peptide receptor 1 (mFPR1). It exhibits nanomolar activity against mFPR1 and micromolar activity against mFPR2. fMIFL is applicable to studies related to bacterial infections.
For research use only. We do not sell to patients.
- Purity: 99.79%
- CAS No.: 112275-26-0
- Formula: C27H42N4O6S
- Molecular Weight:550.71
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
fMIFL (EC50 value; 60 min) potently induces chemotaxis in human neutrophils with an EC50 of 0.30 nM[1].
fMIFL (EC50 value; 15 min) potently induces superoxide anion production in human neutrophils with an EC50 of 1.5 nM[1].
fMIFL (EC50 value) potently induces intracellular calcium mobilization in human neutrophils with an EC50 of 0.09 nM[1].
fMIFL (1 pM-10 μM) potently activates mFPR1 in transfected RBL-2H3 cells, with an EC50 of ~10 nM for calcium mobilization[2].
fMIFL (10 nM-1 μM; 2 min-5 min) induces robust ERK1/2 phosphorylation in mFPR1-transfected RBL-2H3 cells at both 10 nM and 1 μM, with 10 nM fMIFL showing greater potency than 10 nM fMLF[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RBL-2H3 cells stably transfected with mouse formyl peptide receptor 1 (mFPR1)
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Concentration:10 nM, 1 μM (ERK1/2 phosphorylation assay)
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Incubation Time:2 min, 5 min (ERK1/2 phosphorylation assay)
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Result:Induced ERK1/2 phosphorylation to similar extents at 10 nM and 1 μM.
Showed greater potency at 10 nM than fMLF, which required 1 μM for equivalent phosphorylation.
Chemical Information
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CAS No. 112275-26-0
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Appearance Solid
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Molecular Weight 550.71
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Formula C27H42N4O6S
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Color White to off-white
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Sequence
{N-formyl}-Met-Ile-Phe-Leu
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Sequence Shortening
{N-formyl}-MIFL
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : 7.1 mg/mL (12.89 mM; ultrasonic and warming and adjust pH to 8 with NH4OH)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Miyazaki M, et al. Dimeric chemotactic peptides discriminate between chemotaxis and superoxide production of human neutrophils. J Biochem. 1995;117(3):489-494. [Content Brief]
[2]. Southgate EL, et al. Identification of formyl peptides from Listeria monocytogenes and Staphylococcus aureus as potent chemoattractants for mouse neutrophils. J Immunol. 2008;181(2):1429-1437. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.8158 mL | 9.0792 mL | 18.1584 mL | 45.3959 mL |
| 5 mM | 0.3632 mL | 1.8158 mL | 3.6317 mL | 9.0792 mL | |
| 10 mM | 0.1816 mL | 0.9079 mL | 1.8158 mL | 4.5396 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
fMIFL Related Classifications
HY-P10724 Related Classifications
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