2. GPCR/G Protein Anti-infection
  3. Formyl Peptide Receptor (FPR) Bacterial
  4. fMIFL

fMIFL is a formyl tetrapeptide derived from Staphylococcus aureus, and also an agonist of mouse formyl peptide receptor 1 (mFPR1). It exhibits nanomolar activity against mFPR1 and micromolar activity against mFPR2. fMIFL is applicable to studies related to bacterial infections.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

fMIFL

fMIFL Chemical Structure

CAS No. : 112275-26-0

Size Price Stock Quantity
1 mg In-stock
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

fMIFL Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

fMIFL is a formyl tetrapeptide derived from Staphylococcus aureus, and also an agonist of mouse formyl peptide receptor 1 (mFPR1). It exhibits nanomolar activity against mFPR1 and micromolar activity against mFPR2. fMIFL is applicable to studies related to bacterial infections[2].

In Vitro

fMIFL (EC50 value; 60 min) potently induces chemotaxis in human neutrophils with an EC50 of 0.30 nM[1].
fMIFL (EC50 value; 15 min) potently induces superoxide anion production in human neutrophils with an EC50 of 1.5 nM[1].
fMIFL (EC50 value) potently induces intracellular calcium mobilization in human neutrophils with an EC50 of 0.09 nM[1].
fMIFL (1 pM-10 μM) potently activates mFPR1 in transfected RBL-2H3 cells, with an EC50 of ~10 nM for calcium mobilization[2].
fMIFL (10 nM-1 μM; 2 min-5 min) induces robust ERK1/2 phosphorylation in mFPR1-transfected RBL-2H3 cells at both 10 nM and 1 μM, with 10 nM fMIFL showing greater potency than 10 nM fMLF[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

fMIFL Related Antibodies

Western Blot Analysis[2]

Cell Line: RBL-2H3 cells stably transfected with mouse formyl peptide receptor 1 (mFPR1)
Concentration: 10 nM, 1 μM (ERK1/2 phosphorylation assay)
Incubation Time: 2 min, 5 min (ERK1/2 phosphorylation assay)
Result: Induced ERK1/2 phosphorylation to similar extents at 10 nM and 1 μM.
Showed greater potency at 10 nM than fMLF, which required 1 μM for equivalent phosphorylation.
Molecular Weight

550.71

Formula

C27H42N4O6S
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

{N-formyl}-Met-Ile-Phe-Leu
Sequence Shortening

{N-formyl}-MIFL
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 7.1 mg/mL (12.89 mM; ultrasonic and warming and adjust pH to 8 with NH4OH)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8158 mL 9.0792 mL 18.1584 mL
5 mM 0.3632 mL 1.8158 mL 3.6317 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.8158 mL 9.0792 mL 18.1584 mL 45.3959 mL
5 mM 0.3632 mL 1.8158 mL 3.6317 mL 9.0792 mL
10 mM 0.1816 mL 0.9079 mL 1.8158 mL 4.5396 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

fMIFL Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

fMIFL112275-26-0Formyl Peptide Receptor (FPR)Bacterialhuman neutrophilsERK1/2 phosphorylationmouse formyl peptide receptor 1mouse neutrophilsmFPR2Staphylococcus aureusmFPR1superoxide anion productionRBL-2H3 cellschemotaxisInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
fMIFL
Cat. No.:
HY-P10724
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (252 KB) Français - FR (252 KB) Deutsch - DE (252 KB) Norwegian - NO (252 KB) Español - ES (252 KB) Swedish - SV (252 KB) Italian - IT (252 KB) Korean - KR (252 KB) Portuguese - PT (252 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.