FTO-IN-15 

FTO-IN-15 (Compound 8a) is a potent and selective dual-competitive FTO inhibitor with an IC₅₀ of 43.7 nM, showing high selectivity over ALKBH3 and ALKBH5. FTO-IN-15 substantially inhibits FTO demethylation by simultaneously occupying the substrate and 2-oxoglutarate (2-OG) pockets. FTO-IN-15 can be used for the research of acute myeloid leukemia (AML)^[1].

IC₅₀: 1.3 μM^[1]

FTO-IN-15 (compound 8a) (0-50 μM) functions as a competitive inhibitor for the 2-OG binding pocket of FTO, as evidenced by the dose-dependent reversal of its inhibitory activity by increasing concentrations of 2-OG in a cell-free enzymatic assay^[1].
FTO-IN-15 exhibits potent FTO inhibitory activity, with an IC₅₀ of 43.7 nM in a cell-free dot blot assay and 1.3 μM in a PAGE-based demethylation assay^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

466.27

C₂₀H₁₇Cl₂N₃O₆

O=C(/C=C/C(NCCNC(C1=CC(Cl)=C(C(Cl)=C1)NC2=CC=CC=C2C(O)=O)=O)=O)O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Zhang X, et al. Structure-Based Design of a Highly Potent Dual-Competitive FTO Inhibitor for Targeted m6A Demethylase Inhibition in AML. J Med Chem. 2025 Nov 13;68(21):22779-22798.  [Content Brief]