Pleconaril
Based on 1 publication(s) in Google Scholar
Pleconaril is a picornavirus capsid binding inhibitor that prevents attachment and/or virus uncoating. Pleconaril has potential for use in rhinoviruses and enteroviruses research.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.71%
- No. CAS: 153168-05-9
- Fòrmula: C18H18F3N3O3
- Peso molecular:381.35
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
Publications Citing Use of MedChemExpress (MCE) Pleconaril
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Actividad biológica
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Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| H1-HeLa | CC50 |
19.7 μM
Compound: 1
|
Cytotoxicity against human H1HeLa cells assessed as decrease in cell viability after 3 days by MTT assay
Cytotoxicity against human H1HeLa cells assessed as decrease in cell viability after 3 days by MTT assay
|
[PMID: 28581749] |
| H1-HeLa | CC50 |
53.5 μM
Compound: Pleconaril
|
Cytotoxicity in human H1HeLa cells assessed as reduction in cell viability incubated for 3 days by MTT assay
Cytotoxicity in human H1HeLa cells assessed as reduction in cell viability incubated for 3 days by MTT assay
|
[PMID: 28587824] |
| H1-HeLa | CC50 |
19.7 μM
Compound: Pleconaril
|
Cytotoxicity against human H1HeLa cells assessed as reduction in cell viability after 3 days by MTT assay
Cytotoxicity against human H1HeLa cells assessed as reduction in cell viability after 3 days by MTT assay
|
[PMID: 30034598] |
| H1-HeLa | CC50 |
20.8 μM
Compound: Pleconaril
|
Cytotoxicity against human H1-HeLa cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against human H1-HeLa cells assessed as reduction in cell viability by MTT assay
|
[PMID: 35292344] |
| H1-HeLa | CC50 |
20.3 μM
Compound: Pleconaril
|
Cytotoxicity against human H1-HeLa cells infected with 1 MOI human rhinovirus 14 pretreated with compound for 1 hr followed by viral infection measured by MTT assay
Cytotoxicity against human H1-HeLa cells infected with 1 MOI human rhinovirus 14 pretreated with compound for 1 hr followed by viral infection measured by MTT assay
|
[PMID: 38389877] |
| HeLa | CC50 |
12.6 μg/mL
Compound: pleconaril
|
Cytotoxicity against human HeLa cells after 72 hrs
Cytotoxicity against human HeLa cells after 72 hrs
|
[PMID: 18247552] |
| HeLa | IC50 |
0.01 μg/mL
Compound: pleconaril
|
Antiviral activity against HRV2 in HeLa cells
Antiviral activity against HRV2 in HeLa cells
|
[PMID: 18247552] |
| HeLa | IC50 |
0.02 μg/mL
Compound: pleconaril
|
Antiviral activity against HRV14 in HeLa cells
Antiviral activity against HRV14 in HeLa cells
|
[PMID: 18247552] |
| HeLa | IC50 |
0.02 μg/mL
Compound: pleconaril
|
Antiviral activity against HRV16 in HeLa cells
Antiviral activity against HRV16 in HeLa cells
|
[PMID: 18247552] |
| HeLa | IC50 |
0.02 μg/mL
Compound: pleconaril
|
Antiviral activity against HRV1A in HeLa cells
Antiviral activity against HRV1A in HeLa cells
|
[PMID: 18247552] |
| HeLa | EC50 |
224.1 ng/mL
Compound: 1
|
Antirhinoviral activity against Human rhinovirus B infected in human HeLa cells assessed as inhibition of virus-induced cytopathic effect after 5 days by MTT assay
Antirhinoviral activity against Human rhinovirus B infected in human HeLa cells assessed as inhibition of virus-induced cytopathic effect after 5 days by MTT assay
|
[PMID: 24900468] |
| HeLa | EC50 |
23.2 ng/mL
Compound: 1
|
Antirhinoviral activity against minor receptor-resistant Human rhinovirus infected in human HeLa cells assessed as inhibition of virus-induced cytopathic effect after 5 days by MTT assay
Antirhinoviral activity against minor receptor-resistant Human rhinovirus infected in human HeLa cells assessed as inhibition of virus-induced cytopathic effect after 5 days by MTT assay
|
[PMID: 24900468] |
| HeLa | EC50 |
33.8 ng/mL
Compound: 1
|
Antirhinoviral activity against antiviral-resistant Human rhinovirus B infected in human HeLa cells assessed as inhibition of virus-induced cytopathic effect after 5 days by MTT assay
Antirhinoviral activity against antiviral-resistant Human rhinovirus B infected in human HeLa cells assessed as inhibition of virus-induced cytopathic effect after 5 days by MTT assay
|
[PMID: 24900468] |
| HeLa | EC50 |
37.4 ng/mL
Compound: 1
|
Antirhinoviral activity against Human rhinovirus A infected in human HeLa cells assessed as inhibition of virus-induced cytopathic effect after 5 days by MTT assay
Antirhinoviral activity against Human rhinovirus A infected in human HeLa cells assessed as inhibition of virus-induced cytopathic effect after 5 days by MTT assay
|
[PMID: 24900468] |
| HeLa | EC50 |
387.6 ng/mL
Compound: 1
|
Antirhinoviral activity against antiviral-resistant Human rhinovirus A infected in human HeLa cells assessed as inhibition of virus-induced cytopathic effect after 5 days by MTT assay
Antirhinoviral activity against antiviral-resistant Human rhinovirus A infected in human HeLa cells assessed as inhibition of virus-induced cytopathic effect after 5 days by MTT assay
|
[PMID: 24900468] |
| HeLa | EC50 |
49.2 ng/mL
Compound: 1
|
Antirhinoviral activity against major receptor-resistant Human rhinovirus infected in human HeLa cells assessed as inhibition of virus-induced cytopathic effect after 5 days by MTT assay
Antirhinoviral activity against major receptor-resistant Human rhinovirus infected in human HeLa cells assessed as inhibition of virus-induced cytopathic effect after 5 days by MTT assay
|
[PMID: 24900468] |
| HeLa | EC50 |
177 nM
Compound: Pleconaril
|
Antienteroviral activity against Human rhinovirus 14 infected in human HeLa cells assessed as protection against virus-induced cytopathic effect after 2 to 3 days by MTS assay
Antienteroviral activity against Human rhinovirus 14 infected in human HeLa cells assessed as protection against virus-induced cytopathic effect after 2 to 3 days by MTS assay
|
[PMID: 24900715] |
| HeLa | CC50 |
25.9 μM
Compound: 1
|
Cytotoxicity aganist human HeLa cells assessed as decrease in cell viability after 2 days by MTT assay
Cytotoxicity aganist human HeLa cells assessed as decrease in cell viability after 2 days by MTT assay
|
[PMID: 28581749] |
| HeLa | EC50 |
1.4 μM
Compound: 1
|
Antiviral activity against Human poliovirus 3 infected in human HeLa cells assessed as protection against virus induced cytopathic effect after 2 days by MTT assay
Antiviral activity against Human poliovirus 3 infected in human HeLa cells assessed as protection against virus induced cytopathic effect after 2 days by MTT assay
|
[PMID: 28581749] |
| HeLa | CC50 |
42.8 μM
Compound: Pleconaril
|
Cytotoxicity in human HeLa cells assessed as reduction in cell viability incubated for 3 days by MTT assay
Cytotoxicity in human HeLa cells assessed as reduction in cell viability incubated for 3 days by MTT assay
|
[PMID: 28587824] |
| HeLa | EC50 |
>42.8 μM
Compound: Pleconaril
|
Antiviral activity against human coxsackievirus B3 infected in human HeLa cells assessed as reduction in virus-induced cytopathicity incubated for 3 days by MTT assay
Antiviral activity against human coxsackievirus B3 infected in human HeLa cells assessed as reduction in virus-induced cytopathicity incubated for 3 days by MTT assay
|
[PMID: 28587824] |
| HeLa | EC50 |
0.13 μM
Compound: Pleconaril
|
Antiviral activity against human coxsackievirus B1 infected in human HeLa cells assessed as reduction in virus-induced cytopathicity incubated for 3 days by MTT assay
Antiviral activity against human coxsackievirus B1 infected in human HeLa cells assessed as reduction in virus-induced cytopathicity incubated for 3 days by MTT assay
|
[PMID: 28587824] |
| HeLa | EC50 |
0.51 μM
Compound: Pleconaril
|
Antiviral activity against human poliovirus 3 infected in human HeLa cells assessed as reduction in virus-induced cytopathicity incubated for 3 days by MTT assay
Antiviral activity against human poliovirus 3 infected in human HeLa cells assessed as reduction in virus-induced cytopathicity incubated for 3 days by MTT assay
|
[PMID: 28587824] |
| HeLa | CC50 |
131 μM
Compound: Pleconaril
|
Cytotoxicity against human HeLa cells assessed as decrease in cell viability after 72 hrs by MTT assay
Cytotoxicity against human HeLa cells assessed as decrease in cell viability after 72 hrs by MTT assay
|
[PMID: 29039946] |
| HeLa | IC50 |
≤0.18 μM
Compound: 55; WIN63843
|
Cytotoxicity against human HeLa cells after 3 days by MTT assay
Cytotoxicity against human HeLa cells after 3 days by MTT assay
|
[PMID: 31226653] |
| HeLa | CC50 |
26.25 μM
Compound: Pleconaril
|
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs measured by crystal violet based micro plate reader method
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs measured by crystal violet based micro plate reader method
|
[PMID: 31881489] |
| RD | EC50 |
13400 nM
Compound: Pleconaril
|
Antienteroviral activity against Enterovirus 71 infected in human RD cells assessed as protection against virus-induced cytopathic effect after 2 to 3 days by MTS assay
Antienteroviral activity against Enterovirus 71 infected in human RD cells assessed as protection against virus-induced cytopathic effect after 2 to 3 days by MTS assay
|
[PMID: 24900715] |
| RD | CC50 |
>200 μM
Compound: 1
|
Cytotoxicity against human RD cells assessed as inhibition of cell viability by measuring ATP level incubated for 72 hrs by cell titer-glo luminescent cell viability assay
Cytotoxicity against human RD cells assessed as inhibition of cell viability by measuring ATP level incubated for 72 hrs by cell titer-glo luminescent cell viability assay
|
[PMID: 36254462] |
| Vero | EC50 |
1084 nM
Compound: Pleconaril
|
Antienteroviral activity against poliovirus 2 infected in african green monkey Vero cells assessed as protection against virus-induced cytopathic effect after 2 to 3 days by MTS assay
Antienteroviral activity against poliovirus 2 infected in african green monkey Vero cells assessed as protection against virus-induced cytopathic effect after 2 to 3 days by MTS assay
|
[PMID: 24900715] |
| Vero | EC50 |
24200 nM
Compound: Pleconaril
|
Antienteroviral activity against poliovirus 1 infected in african green monkey Vero cells assessed as protection against virus-induced cytopathic effect after 2 to 3 days by MTS assay
Antienteroviral activity against poliovirus 1 infected in african green monkey Vero cells assessed as protection against virus-induced cytopathic effect after 2 to 3 days by MTS assay
|
[PMID: 24900715] |
| Vero | EC50 |
33 nM
Compound: Pleconaril
|
Antienteroviral activity against Coxsackievirus A9 infected in african green monkey Vero cells assessed as protection against virus-induced cytopathic effect after 2 to 3 days by MTS assay
Antienteroviral activity against Coxsackievirus A9 infected in african green monkey Vero cells assessed as protection against virus-induced cytopathic effect after 2 to 3 days by MTS assay
|
[PMID: 24900715] |
| Vero | EC50 |
84 nM
Compound: Pleconaril
|
Antienteroviral activity against poliovirus 3 infected in african green monkey Vero cells assessed as protection against virus-induced cytopathic effect after 2 to 3 days by MTS assay
Antienteroviral activity against poliovirus 3 infected in african green monkey Vero cells assessed as protection against virus-induced cytopathic effect after 2 to 3 days by MTS assay
|
[PMID: 24900715] |
| Vero | EC50 |
13 μM
Compound: Pleconaril
|
Antienteroviral activity against Coxsackievirus B3 infected in african green monkey Vero cells assessed as protection against virus-induced cytopathic effect after 2 to 3 days by MTS assay
Antienteroviral activity against Coxsackievirus B3 infected in african green monkey Vero cells assessed as protection against virus-induced cytopathic effect after 2 to 3 days by MTS assay
|
[PMID: 24900715] |
| Vero | EC50 |
0.005 μM
Compound: Pleconaril
|
Antiviral activity against Coxsackievirus B5 infected in African green monkey Vero 76 cells after 3 days by plaque reduction assay
Antiviral activity against Coxsackievirus B5 infected in African green monkey Vero 76 cells after 3 days by plaque reduction assay
|
[PMID: 25913116] |
| Vero | EC50 |
2.2 μM
Compound: Pleconaril
|
Antiviral activity against Poliovirus Sb-1 infected in African green monkey Vero 76 cells after 2 days by plaque reduction assay
Antiviral activity against Poliovirus Sb-1 infected in African green monkey Vero 76 cells after 2 days by plaque reduction assay
|
[PMID: 25913116] |
| Vero | IC50 |
0.0025 μM
Compound: pleconaril
|
Antiviral activity against Coxsackievirus B3 infected in African green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect
Antiviral activity against Coxsackievirus B3 infected in African green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect
|
[PMID: 26110443] |
| Vero | IC50 |
0.001 μM
Compound: Pleconarild
|
Antiviral activity against Coxsackievirus B3 infected in African green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect
Antiviral activity against Coxsackievirus B3 infected in African green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect
|
[PMID: 27792321] |
| Vero | CC50 |
>1.05 μM
Compound: Pleconaril
|
Cytotoxicity in African green monkey Vero cells assessed as reduction in cell viability
Cytotoxicity in African green monkey Vero cells assessed as reduction in cell viability
|
[PMID: 32378892] |
| Vero | EC50 |
0.002 μM
Compound: Pleconaril
|
Antiviral activity against Coxsackievirus B3 infected in Vero cells assessed as reduction in virus-induced cytopathic effect incubated for 48 hrs by Reed-Muench method
Antiviral activity against Coxsackievirus B3 infected in Vero cells assessed as reduction in virus-induced cytopathic effect incubated for 48 hrs by Reed-Muench method
|
[PMID: 32378892] |
| Vero 76 | CC50 |
80 μM
Compound: Pleconaril
|
Cytotoxicity against African green monkey Vero 76 cells assessed as cell viability after 48 to 96 hrs by MTT method
Cytotoxicity against African green monkey Vero 76 cells assessed as cell viability after 48 to 96 hrs by MTT method
|
[PMID: 25913116] |
| Vero 76 | CC50 |
70 μM
Compound: Pleconaril
|
Cytotoxicity against african green monkey Vero 76 cells assessed as cell viability after 48 to 96 hrs by crystal violet staining method
Cytotoxicity against african green monkey Vero 76 cells assessed as cell viability after 48 to 96 hrs by crystal violet staining method
|
[PMID: 26443549] |
| Vero 76 | EC50 |
0.008 μM
Compound: Pleconaril
|
Antiviral activity against human CVB5 infected in african green monkey Vero76 cells assessed as inhibition of virus-induced cytopathogenicity after 3 days by plaque reduction assay
Antiviral activity against human CVB5 infected in african green monkey Vero76 cells assessed as inhibition of virus-induced cytopathogenicity after 3 days by plaque reduction assay
|
[PMID: 26443549] |
| Vero 76 | EC50 |
2 μM
Compound: Pleconaril
|
Antiviral activity against Human poliovirus 1 Sabin infected in african green monkey Vero76 cells assessed as inhibition of virus-induced cytopathogenicity by plaque reduction assay
Antiviral activity against Human poliovirus 1 Sabin infected in african green monkey Vero76 cells assessed as inhibition of virus-induced cytopathogenicity by plaque reduction assay
|
[PMID: 26443549] |
| Vero 76 | CC50 |
83 μM
Compound: Pleconaril
|
Cytotoxicity against African green monkey Vero 76 cells assessed as decrease in cell viability after 48 to 96 hrs by MTT assay
Cytotoxicity against African green monkey Vero 76 cells assessed as decrease in cell viability after 48 to 96 hrs by MTT assay
|
[PMID: 29028528] |
| Vero 76 | CC50 |
>100 μM
Compound: Pleconaril
|
Cytotoxicity against African green monkey Vero 76 cells assessed as reduction in cell viability after 48 to 96 hrs by MTT assay
Cytotoxicity against African green monkey Vero 76 cells assessed as reduction in cell viability after 48 to 96 hrs by MTT assay
|
[PMID: 29339251] |
Pleconaril (0.4-50 µM, 2 days) produces substantial cytotoxicity at 50 μM concentrations in RD cells[2].
Pleconaril (0.001-4 µg/mL, 24 h) increases the cell viability of RD cells infected with EV71[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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No. CAS 153168-05-9
-
Appearance Solid
-
Peso molecular 381.35
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Fòrmula C18H18F3N3O3
-
Color White to off-white
-
SMILES
FC(C1=NC(C2=CC(C)=C(OCCCC3=CC(C)=NO3)C(C)=C2)=NO1)(F)F
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Synonyms
VP 63843; Win 63843
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
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Journal Impact Factor
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Most Recent
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iScience
Quantitative virus infectivity titration and dose-response analyses using cell-culture based methods and python code-assisted data analysis. [Abstract]2026 Feb 13;29(3):115018. PMID: 41809041
Solvente y solubilidad
DMSO : 100 mg/mL (262.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.56 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (271 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Benschop KS, et al. Genetic and antigenic structural characterization for resistance of echovirus 11 to pleconaril in an immunocompromised patient. J Gen Virol. 2015 Mar;96(Pt 3):571-9. [Content Brief]
[2]. Lee JS, et al. Antiviral Activity of Itraconazole against Echovirus 30 Infection In Vitro. Osong Public Health Res Perspect. 2017 Oct;8(5):318-324. [Content Brief]
[3]. Zhang G, et al. In vitro and in vivo evaluation of ribavirin and pleconaril antiviral activity against enterovirus 71 infection. Arch Virol. 2012 Apr;157(4):669-79. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6223 mL | 13.1113 mL | 26.2226 mL | 65.5566 mL |
| 5 mM | 0.5245 mL | 2.6223 mL | 5.2445 mL | 13.1113 mL | |
| 10 mM | 0.2622 mL | 1.3111 mL | 2.6223 mL | 6.5557 mL | |
| 15 mM | 0.1748 mL | 0.8741 mL | 1.7482 mL | 4.3704 mL | |
| 20 mM | 0.1311 mL | 0.6556 mL | 1.3111 mL | 3.2778 mL | |
| 25 mM | 0.1049 mL | 0.5245 mL | 1.0489 mL | 2.6223 mL | |
| 30 mM | 0.0874 mL | 0.4370 mL | 0.8741 mL | 2.1852 mL | |
| 40 mM | 0.0656 mL | 0.3278 mL | 0.6556 mL | 1.6389 mL | |
| 50 mM | 0.0524 mL | 0.2622 mL | 0.5245 mL | 1.3111 mL | |
| 60 mM | 0.0437 mL | 0.2185 mL | 0.4370 mL | 1.0926 mL | |
| 80 mM | 0.0328 mL | 0.1639 mL | 0.3278 mL | 0.8195 mL | |
| 100 mM | 0.0262 mL | 0.1311 mL | 0.2622 mL | 0.6556 mL |