Casein
Based on 2 publication(s) in Google Scholar
Casein is an orally active phosphoprotein that can be separated into various electrophoretic components, such as α2-Casein, κ-Casein, β-casein, and γ-casein. Casein has also been blended and grafted with other polymers, cross-linkers, or monomers to improve its functional properties. Casein enhances calcium absorption and reduces the extent of fissure as well as smooth-surface caries. Casein promotes proliferation of prostate cancer. Casein has various applications in the paper, leather, textile, and food industries, serving as coatings, adhesives, and packaging materials.
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- Pureza : 97.40%
- No. CAS: 9000-71-9
- Fòrmula: C81H125N22O39P
- Peso molecular:2061.96
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Chemical Information
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Actividad biológica
Descripciòn
In Vitro
Casein (1 mg/mL) promotes proliferation of prostate cancer cells PC3 (166%) and LNCaP (142%)[2].
Casein (0.2%) supports cell density-dependent growth of Myxococcus xanthus[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
Casein (500 ppm, p.o., 2 weeks) enhances calcium absorption in the rat[5].
Casein (2% (w/v), in drinking water, p.o.) reduces the extent of fissure and smooth-surface caries of male Sprague-Dawley rats consuming a solid cariogenic diet[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Ensayo clínico
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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No. CAS 9000-71-9
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Appearance Solid
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Peso molecular 2061.96
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Fòrmula C81H125N22O39P
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Color Off-white to yellow
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SMILES
[Casein]
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (2)
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Journal Impact Factor
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Most Recent
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Int J Biol Macromol
Dynamic catalytic domain plasticity governs substrate specificity in industrial serine proteases: Structural and functional implications. [Abstract]2025 Oct 17;331(Pt 1):148374. PMID: 41110577 -
Talanta
Phenylboronic acid decorated SiO2@Fe3O4 magnetic nanoparticle coupled with peroxidase-like Au,Pt,Cu@polydopamine immunoprobe based sandwich approach for the detection of transferrin. [Abstract]2025 Aug 26;297(Pt B):128747. PMID: 40885136
Casein purchased from MedChemExpress. Usage Cited in: Talanta. 2025 Aug 26;297(Pt B):128747. [Abstract]
Adsorption performance of PBA-SiO2@Fe3O4 toward TRF after blocking with different concentrations of Casein (0.1 %-1 %).
Casein purchased from MedChemExpress. Usage Cited in: Talanta. 2025 Aug 26;297(Pt B):128747. [Abstract]
Adsorption performance of PBA-SiO2@Fe3O4 toward TRF after blocking with Casein (1 %).
Casein purchased from MedChemExpress. Usage Cited in: Talanta. 2025 Aug 26;297(Pt B):128747. [Abstract]
Adsorption performance of PBA-SiO2@Fe3O4 toward various glycoproteins utilizingCasein (1 %).
Casein purchased from MedChemExpress. Usage Cited in: Talanta. 2025 Aug 26;297(Pt B):128747. [Abstract]
Adsorption performance of PBA-SiO2@Fe3O4 toward various glycoproteins utilizingCasein (0.1 %-1 %).
Casein purchased from MedChemExpress. Usage Cited in: Talanta. 2025 Aug 26;297(Pt B):128747. [Abstract]
Casein (0.1 %; 400 μL) did not occupy the PBA binding sites responsible for interaction with sugar residues in TRF, as suggested by the negligible impact of increasing glucose concentrations on its blocking effect.
Protocolo
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Kinase activity and phosphorylation assays
Kinase activity assays measure the ability of kinases to transfer phosphate groups from ATP to specific substrates, while phosphorylation assays detect the presence and levels of phosphorylated proteins. Common methods include radiolabeled ATP incorporation (e. g. ,), ADP release detection via bioluminescence (e. g. ,[3]), enzyme-linked immunosorbent assays (ELISA) for phospho-specific epitopes (e. g. ,[6]), and microtiter-based formats for high-throughput screening (e. g. ,[8]). The ADP-Glo assay quantifies kinase activity by measuring ADP produced during phosphorylation using a luciferase-based system. Radiometric assays involve autoradiography or scintillation counting after incorporation of 32P-labeled ATP into substrate proteins. ELISA-based approaches rely on phospho-specific antibodies to detect activated kinases in cell lysates or purified samples.
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Protocol for Kinase activity and phosphorylation assays
Kinase activity assays measure transfer of phosphate from ATP to a protein or peptide substrate, generating phosphorylated substrate, ADP, or incorporated radiolabeled phosphate as the readout; phosphorylation assays measure site-specific phosphorylation in cells or tissues as a proxy for kinase-pathway activation, inhibition, or substrate regulation. Phosphorylation can be detected by phospho-specific Western blot, immunoprecipitation kinase assay, phospho-immunofluorescence, phospho-flow cytometry, luminescent ADP detection, radiolabeled ATP incorporation, or reporter-based pathway assays, and these readouts can be applied to cancer cells, primary neurons, mouse tumors, organoids, inflammatory macrophages, ferroptosis studies, and mitophagy studies when the kinase target is biologically relevant.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureza y Documentación
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Ficha de datos (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
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- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Ghosh A, et al. Effect of cross-linking on microstructure and physical performance of casein protein. Biomacromolecules. 2009;10(7):1681-1688. [Content Brief]
[2]. Park SW, et al. A milk protein, casein, as a proliferation promoting factor in prostate cancer cells. World J Mens Health. 2014 Aug;32(2):76-82. [Content Brief]
[3]. Rosenberg E, et al. Cell density-dependent growth of Myxococcus xanthus on casein. J Bacteriol. 1977 Feb;129(2):770-7. [Content Brief]
[4]. Lillefosse HH, et al. Hydrolyzed casein reduces diet-induced obesity in male C57BL/6J mice. J Nutr. 2013 Sep;143(9):1367-75. [Content Brief]
[5]. Bennett T, et al. The effect of high intakes of casein and casein phosphopeptide on calcium absorption in the rat. Br J Nutr. 2000 Jun;83(6):673-80. [Content Brief]
[6]. Reynolds EC, et al. Effect of casein and whey-protein solutions on caries experience and feeding patterns of the rat. Arch Oral Biol. 1984;29(11):927-33. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Casein Related Classifications
HY-125865 Related Classifications
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