Glycerol-13C-1
Based on 1 Customer Validation
Glycerol-13C-1 (Trihydroxypropane-13C-1) is the 13C-labeled Glycerol (HY-B1659). Glycerol is used in sample preparation and gel formation for polyacrylamide gel electrophoresis.
For research use only. We do not sell to patients.
- CAS No.: 82425-96-5
- Formula: C213CH8O3
- Molecular Weight:93.09
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
Description
In Vitro
Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Application
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
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CAS No. 82425-96-5
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Unlabeled CAS 56-81-5
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Appearance Liquid
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Molecular Weight 93.09
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Formula C213CH8O3
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SMILES
OC[13CH](O)CO
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Synonyms
Trihydroxypropane-13C-1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (537.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Polyacrylamide gel electrophoresis (PAGE) (<1 kb)
Gel electrophoresis is a method for separating biological macromolecules (such as nucleic acids or proteins) by forcing them through a gel matrix under an electric field.
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Protocol for Protein Electrophoresis
Protein electrophoresis separates proteins in a polyacrylamide gel under an electric field; in SDS-PAGE, sodium dodecyl sulfate denatures proteins and gives them a broadly similar negative charge-to-mass ratio, so migration mainly reflects apparent molecular mass through the gel matrix. The readout is a stained protein band pattern: band position estimates apparent molecular mass using protein standards, band intensity reflects relative protein abundance within the linear range of staining/detection, and changes in band pattern can reflect protein expression, degradation, purification, or sample composition.
Purity & Documentation
References
[1]. Pennings S, et al. Effect of glycerol on the separation of nucleosomes and bent DNA in low ionic strengthpolyacrylamide gel electrophoresis. Nucleic Acids Res. 1992 Dec 25;20(24):6667-72. [Content Brief]
[2]. Yazdani SS, et al. Anaerobic fermentation of glycerol: a path to economic viability for the biofuelsindustry. Curr Opin Biotechnol. 2007 Jun;18(3):213-9. [Content Brief]
[3]. Huang ZH, et al. Expression and function of P-glycoprotein in rats with glycerol-induced acute renal failure. Eur J Pharmacol. 2000 Oct 20;406(3):453-60. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 10.7423 mL | 53.7115 mL | 107.4229 mL | 268.5573 mL |
| 5 mM | 2.1485 mL | 10.7423 mL | 21.4846 mL | 53.7115 mL | |
| 10 mM | 1.0742 mL | 5.3711 mL | 10.7423 mL | 26.8557 mL | |
| 15 mM | 0.7162 mL | 3.5808 mL | 7.1615 mL | 17.9038 mL | |
| 20 mM | 0.5371 mL | 2.6856 mL | 5.3711 mL | 13.4279 mL | |
| 25 mM | 0.4297 mL | 2.1485 mL | 4.2969 mL | 10.7423 mL | |
| 30 mM | 0.3581 mL | 1.7904 mL | 3.5808 mL | 8.9519 mL | |
| 40 mM | 0.2686 mL | 1.3428 mL | 2.6856 mL | 6.7139 mL | |
| 50 mM | 0.2148 mL | 1.0742 mL | 2.1485 mL | 5.3711 mL | |
| 60 mM | 0.1790 mL | 0.8952 mL | 1.7904 mL | 4.4760 mL | |
| 80 mM | 0.1343 mL | 0.6714 mL | 1.3428 mL | 3.3570 mL | |
| 100 mM | 0.1074 mL | 0.5371 mL | 1.0742 mL | 2.6856 mL |
Glycerol-13C-1 Related Classifications
HY-B1659S12 Related Classifications
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