H-L-Lys(4-N3-Z)-OH hydrochloride
Based on 1 Customer Validation
H-L-Lys (4-N3-Z)-OH hydrochloride is an unnatural lysine analog and a substrate of the PABKRS mutant PylRS. H-L-Lys (4-N3-Z)-OH hydrochloride enables site-specific genetic integration into proteins via amber stop codon suppression; it undergoes Staudinger reduction with phosphine compounds, followed by a self-elimination reaction to release native lysine. H-L-Lys (4-N3-Z)-OH hydrochloride serves as a genetically encoded element for the temporal chemical control of protein SUMOylation and nuclear translocation in living mammalian cells, and also acts as a bioorthogonal reaction handle for bioconjugation reactions.
For research use only. We do not sell to patients.
- Purity : 99.69%
- CAS No.: 2084913-49-3
- Formula: C14H20ClN5O4
- Molecular Weight:357.79
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
H‑L‑Lys (4‑N3‑Z)‑OH (PABK) hydrochloride exhibits a deprotection reaction with a t95 of 17.7 min[1].
H-L-Lys (4-N3-Z)-OH hydrochloride is efficiently incorporated into sfGFP-Y151TAG to yield unmodified sfGFP-Y151PABK in Escherichia coli expressing the PABKRS synthetase[1].
H-L-Lys (4-N3-Z)-OH hydrochloride is efficiently incorporated into the mCherry-TAG-EGFP-HA reporter gene in transfected HEK293T cells to produce full-length fusion proteins at high yields[1].
H-L-Lys (4-N3-Z)-OH hydrochloride mediates phosphine-triggered nuclear translocation, with a t1/2 of 26.7 min in the presence of 100 μM 2DPBM (complete translocation is achieved at 75 min)[1].
H-L-Lys (4-N3-Z)-OH hydrochloride enables the quantitative conversion of unmodified RGΔN to its 37 kDa SUMOylated form via the endogenous cellular SUMOylation pathway through a phosphine-triggered deprotection reaction in HEK293T cells expressing FLAG-RGΔN-K526TAG[1].
H‑L‑Lys (4‑N3‑Z)‑OH hydrochloride enables phosphine‑mediated activation of RanGAP1‑K524PABK‑RFP and restores native SUMOylation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2084913-49-3
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Appearance Solid
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Molecular Weight 357.79
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Formula C14H20ClN5O4
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Color White to light yellow
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SMILES
OC([C@@H](N)CCCCNC(OCC1=CC=C(C=C1)N=[N+]=[N-])=O)=O.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
In Vitro:
Methanol : 5 mg/mL (13.97 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Methanol | 1 mM | 2.7949 mL | 13.9747 mL | 27.9494 mL | 69.8734 mL |
| 5 mM | 0.5590 mL | 2.7949 mL | 5.5899 mL | 13.9747 mL | |
| 10 mM | 0.2795 mL | 1.3975 mL | 2.7949 mL | 6.9873 mL |
H-L-Lys(4-N3-Z)-OH hydrochloride Related Classifications
HY-151761 Related Classifications
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