2. JAK/STAT Signaling
  3. Pim
  4. HJ-PI01

HJ-PI01  (Synonyms: 10-Acetylphenoxazine)

Cat. No.: HY-129163 Purity: 99.72%
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HJ-PI01 (10-Acetylphenoxazine) is an orally active Pim-2 inhibitor. HJ-PI01 induces apoptosis and autophagic cell death of cancer cells. HJ-PI01 inhibits tumor growth in MDA-MB-231 xenograft mice. HJ-PI01 can be used for cancer research.

For research use only. We do not sell to patients.

HJ-PI01 Chemical Structure

HJ-PI01 Chemical Structure

CAS No. : 6192-43-4

Size Price Stock Quantity
5 mg USD 150 In-stock
10 mg USD 250 In-stock
25 mg USD 550 In-stock
50 mg USD 850 In-stock
100 mg USD 1350 In-stock
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Based on 1 publication(s) in Google Scholar

HJ-PI01 Related Antibodies

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Description

HJ-PI01 (10-Acetylphenoxazine) is an orally active Pim-2 inhibitor. HJ-PI01 induces apoptosis and autophagic cell death of cancer cells. HJ-PI01 inhibits tumor growth in MDA-MB-231 xenograft mice. HJ-PI01 can be used for cancer research[1].

In Vitro

HJ-PI01 (0-3200 nmol/L; 48 hours) dose-dependently inhibits MDA-MB-231 cell growth and shows a substantial improvement compared with chlorpromazine and PI003[1].
HJ-PI01 (100-400 nmol/L; 24 hours) shows weak toxicity to normal non-cancer cells[1].
HJ-PI01 (300 nmol/L; 24 hours) induces autophagic cell death of MDA-MB-231 cells[1].
HJ-PI01 (300 nmol/L; 24 hours) induces apoptotic cell death in MDA-MB-231 cells[1].
HJ-PI01 (300 and 460 nmol/L; 12-48 hours) affects the expression levels of autophagy and apoptosis-related proteins[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HJ-PI01 Related Antibodies

Western Blot Analysis[1]

Cell Line: MDA-MB-231 cell line
Concentration: 300 and 460 nmol/L
Incubation Time: 12, 24, 36 and 48 hours
Result: Time-dependently increased LC3-II and Beclin-1 and induced p62 degradation in MDA-MB-231 cells. Increased the level of Bax. Decreased the level of Bcl-2, and Pim-2 and Pim-2 phosphorylation. Activated caspase-9 and caspase-3.
In Vivo

HJ-PI01 (40 mg/kg; oral administration, once daily for 10 days) inhibits tumor growth in MDA-MB-231 xenograft mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c female nude mice with MDA-MB-231 cells injection[1]
Dosage: 40 mg/kg
Administration: Oral administration; 40 mg/kg, once daily for 10 days
Result: Significantly inhibited tumor growth with an obvious decreasing of the body, liver, spleen and kidney weights of the mice.
Molecular Weight

225.24

Formula

C14H11NO2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(N1C2=C(C=CC=C2)OC3=CC=CC=C13)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (221.99 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4397 mL 22.1985 mL 44.3971 mL
5 mM 0.8879 mL 4.4397 mL 8.8794 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

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C2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.4397 mL 22.1985 mL 44.3971 mL 110.9927 mL
5 mM 0.8879 mL 4.4397 mL 8.8794 mL 22.1985 mL
10 mM 0.4440 mL 2.2199 mL 4.4397 mL 11.0993 mL
15 mM 0.2960 mL 1.4799 mL 2.9598 mL 7.3995 mL
20 mM 0.2220 mL 1.1099 mL 2.2199 mL 5.5496 mL
25 mM 0.1776 mL 0.8879 mL 1.7759 mL 4.4397 mL
30 mM 0.1480 mL 0.7400 mL 1.4799 mL 3.6998 mL
40 mM 0.1110 mL 0.5550 mL 1.1099 mL 2.7748 mL
50 mM 0.0888 mL 0.4440 mL 0.8879 mL 2.2199 mL
60 mM 0.0740 mL 0.3700 mL 0.7400 mL 1.8499 mL
80 mM 0.0555 mL 0.2775 mL 0.5550 mL 1.3874 mL
100 mM 0.0444 mL 0.2220 mL 0.4440 mL 1.1099 mL
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HJ-PI01 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HJ-PI016192-43-410-AcetylphenoxazinePimPim kinasesapoptosiscell deathautophagyMDA-MB-231LC3-IIBeclin-1p62BaxInhibitorinhibitorinhibit

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