Bromophenol blue
Based on 1 Customer Validation
Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. Bromophenol blue is used as a sensor for the determination of several compounds including ammonia, drugs, proteins and amino acids. The binding of Bromophenol blue to proteins is accompanied by the appearance of a strong absorbance at 610 nm.
For research use only. We do not sell to patients.
- CAS No.: 115-39-9
- Formula: C19H10Br4O5S
- Molecular Weight:669.96
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Storage:
RT, stored under nitrogen.
In solvent -80°C, 1 year , -20°C, 6 months
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of Bromophenol blue Staining Solution:[2]
Prepare Bromophenol blue staining solution with DMSO.
2. Protein assay:[2]
2.1 Proteins were dissolved in distilled water, and their concentrations were adjusted to 1 mg/mL with the exceptions of the myoglobin solution which was prepared gravimetrically, and the y-globulin solution, which was made according to the suppliers.
2.2 For performing the protein assay, protein solutions containing from 10 to 80 μg of protein in 0.1 mL were mixed with 0.9 mL of the Bromophenol blue solution.
2.3 The absorbance at 610 nm was determined using a blank made by mixing 0.1 mL of distilled water and 0.9 mL of the dye solution.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 115-39-9
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Appearance Solid
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Molecular Weight 669.96
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Formula C19H10Br4O5S
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Color Orange to red
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SMILES
O=S1(OC(C2=CC(Br)=C(O)C(Br)=C2)(C3=CC(Br)=C(O)C(Br)=C3)C4=CC=CC=C41)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
RT, stored under nitrogen
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
DMSO : 50 mg/mL (74.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (282 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Dangui AZ, et, al. Preferential solvation bromophenol blue in water-alcohol binary mixture. Spectrochim Acta A Mol Biomol Spectrosc. 2018 Oct 5; 203: 333-341. [Content Brief]
[2]. Flores R. A rapid and reproducible assay for quantitative estimation of proteins using bromophenol blue. Anal Biochem. 1978 Aug 1;88(2):605-11. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4926 mL | 7.4631 mL | 14.9263 mL | 37.3157 mL |
| 5 mM | 0.2985 mL | 1.4926 mL | 2.9853 mL | 7.4631 mL | |
| 10 mM | 0.1493 mL | 0.7463 mL | 1.4926 mL | 3.7316 mL | |
| 15 mM | 0.0995 mL | 0.4975 mL | 0.9951 mL | 2.4877 mL | |
| 20 mM | 0.0746 mL | 0.3732 mL | 0.7463 mL | 1.8658 mL | |
| 25 mM | 0.0597 mL | 0.2985 mL | 0.5971 mL | 1.4926 mL | |
| 30 mM | 0.0498 mL | 0.2488 mL | 0.4975 mL | 1.2439 mL | |
| 40 mM | 0.0373 mL | 0.1866 mL | 0.3732 mL | 0.9329 mL | |
| 50 mM | 0.0299 mL | 0.1493 mL | 0.2985 mL | 0.7463 mL | |
| 60 mM | 0.0249 mL | 0.1244 mL | 0.2488 mL | 0.6219 mL |
Bromophenol blue Related Classifications
HY-B1571 Related Classifications
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