2. Others
  3. Fluorescent Dye
  4. HKOCl-4

HKOCl-4 (BXY2142) is a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid with excellent sensitivity and selectivity. HKOCl-4 has longer absorption wavelength and better pH stability compared with fluorescein-based probes. Ex: 530 nm; Em 557 nm.

For research use only. We do not sell to patients.

HKOCl-4

HKOCl-4 Chemical Structure

CAS No. : 2031170-85-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 mg In-stock
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

HKOCl-4 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE HKOCl-4

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

HKOCl-4 (BXY2142) is a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid with excellent sensitivity and selectivity[1]. HKOCl-4 has longer absorption wavelength and better pH stability compared with fluorescein-based probes. Ex: 530 nm; Em 557 nm.

IC50 & Target

Ex: 530 nm; Em 557 nm
In Vitro

HKOCl-4 (10 μM) shows an increasing florescence intensity with increasing concentration of hypochlorous acid[1].
HKOCl-4 exhibits a ultra-fast response towards 1 equiv of HOCl (reached a plateau within 2 min) , and it shows a >20-fold increase in fluorescence intensity over other analytes (1 equiv of OH and ONOO as well as 10 equiv of O2, NO, 1O2, ROO , TBHP and H2O2)[2].
HKOCl-4 is first tested and it was proven to be non-toxic in living cells even at a concentration up to 50 μM in RAW264.7 macrophages[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HKOCl-4 Related Antibodies
Molecular Weight

688.59

Formula

C38H35Cl2NO7
CAS No.
Appearance

Solid

Color

Yellow to orange

Emission (Em)

557
Excitation (Ex)

530
SMILES

O=C(OCC)CCCN1C(C)(C)CC(C)C2=C1C=C3C(C4(OC(C5=C4C=CC=C5)=O)C6=CC=C(OC7=CC(Cl)=C(O)C(Cl)=C7)C=C6O3)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 200 mg/mL (290.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4522 mL 7.2612 mL 14.5224 mL
5 mM 0.2904 mL 1.4522 mL 2.9045 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4522 mL 7.2612 mL 14.5224 mL 36.3061 mL
5 mM 0.2904 mL 1.4522 mL 2.9045 mL 7.2612 mL
10 mM 0.1452 mL 0.7261 mL 1.4522 mL 3.6306 mL
15 mM 0.0968 mL 0.4841 mL 0.9682 mL 2.4204 mL
20 mM 0.0726 mL 0.3631 mL 0.7261 mL 1.8153 mL
25 mM 0.0581 mL 0.2904 mL 0.5809 mL 1.4522 mL
30 mM 0.0484 mL 0.2420 mL 0.4841 mL 1.2102 mL
40 mM 0.0363 mL 0.1815 mL 0.3631 mL 0.9077 mL
50 mM 0.0290 mL 0.1452 mL 0.2904 mL 0.7261 mL
60 mM 0.0242 mL 0.1210 mL 0.2420 mL 0.6051 mL
80 mM 0.0182 mL 0.0908 mL 0.1815 mL 0.4538 mL
100 mM 0.0145 mL 0.0726 mL 0.1452 mL 0.3631 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

HKOCl-4 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HKOCl-42031170-85-9BXY2142HKOCl4HKOCl 4BXY 2142BXY-2142Fluorescent DyeHOClrhodol-basedFluorescent Probehypochlorous acidO2NO1O2ROO-TBHPInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
HKOCl-4
Cat. No.:
HY-130027
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (252 KB) Français - FR (252 KB) Deutsch - DE (252 KB) Norwegian - NO (252 KB) Español - ES (252 KB) Swedish - SV (252 KB) Italian - IT (252 KB) Korean - KR (252 KB) Portuguese - PT (252 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.