Homopropargylglycine
Based on 1 Customer Validation
Homopropargylglycine is a Methionine (HY-13694) analogue. Homopropargylglycine is incorporated at a suitable rate into newly synthesized proteins in a variety of systems, including mammalian cell culture. Homopropargylglycine can be used to identify old and new protein populations. Homopropargylglycine is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- Purity : 98.54%
- CAS No.: 215160-72-8
- Formula: C6H9NO2
- Molecular Weight:127.14
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Homopropargylglycine enables Bio-Orthogonal Non-Canonical Amino acid Tagging (BONCAT) of a small sample of the proteins being synthesized in Arabidopsis plants or cell cultures, facilitating their click-chemistry enrichment for analysis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 215160-72-8
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Appearance Solid
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Molecular Weight 127.14
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Formula C6H9NO2
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Color White to yellow
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SMILES
C#CCCC(N)C(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
H2O : 100 mg/mL (786.53 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
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Somatic Cell Culture
A method of simulating the in vivo environment in vitro to maintain the cell growth, differentation and main functions.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 7.8653 mL | 39.3267 mL | 78.6535 mL | 196.6336 mL |
| 5 mM | 1.5731 mL | 7.8653 mL | 15.7307 mL | 39.3267 mL | |
| 10 mM | 0.7865 mL | 3.9327 mL | 7.8653 mL | 19.6634 mL | |
| 15 mM | 0.5244 mL | 2.6218 mL | 5.2436 mL | 13.1089 mL | |
| 20 mM | 0.3933 mL | 1.9663 mL | 3.9327 mL | 9.8317 mL | |
| 25 mM | 0.3146 mL | 1.5731 mL | 3.1461 mL | 7.8653 mL | |
| 30 mM | 0.2622 mL | 1.3109 mL | 2.6218 mL | 6.5545 mL | |
| 40 mM | 0.1966 mL | 0.9832 mL | 1.9663 mL | 4.9158 mL | |
| 50 mM | 0.1573 mL | 0.7865 mL | 1.5731 mL | 3.9327 mL | |
| 60 mM | 0.1311 mL | 0.6554 mL | 1.3109 mL | 3.2772 mL | |
| 80 mM | 0.0983 mL | 0.4916 mL | 0.9832 mL | 2.4579 mL | |
| 100 mM | 0.0787 mL | 0.3933 mL | 0.7865 mL | 1.9663 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Homopropargylglycine Related Classifications
HY-W259668 Related Classifications
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