1. NF-κB Cell Cycle/DNA Damage Anti-infection Apoptosis
  2. NF-κB DNA/RNA Synthesis Parasite Apoptosis
  3. Isochamaejasmin

Isochamaejasmin is a biflavonoid with anti-cancer, antiplasmodial and insecticidal activities. Isochamaejasmin displays a potent NF-κB (NF-κB) activation activity. Isochamaejasmin could cause DNA damage and induce apoptosis via the mitochondrial pathway in AW1 cells. Isochamaejasmin also has a moderate antiplasmodial activity (IC50 of 7.3 μM for P. falciparum) and relatively low cytotoxicity (CC50 of 29.0 μM).

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Isochamaejasmin Chemical Structure

Isochamaejasmin Chemical Structure

CAS No. : 93859-63-3

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Description

Isochamaejasmin is a biflavonoid with anti-cancer, antiplasmodial and insecticidal activities. Isochamaejasmin displays a potent NF-κB (NF-κB) activation activity. Isochamaejasmin could cause DNA damage and induce apoptosis via the mitochondrial pathway in AW1 cells[1][2]. Isochamaejasmin also has a moderate antiplasmodial activity (IC50 of 7.3 μM for P. falciparum) and relatively low cytotoxicity (CC50 of 29.0 μM)[3].

In Vitro

Isochamaejasmin (6.25-100 μM; 24-72 h) shows potential toxicity against AW1 cells via time- and dose-dependent manners. Isochamaejasmin (1000 mg/L, 500 mg/L, 250 mg/L, 125 mg/L, 62.5 mg/L; 24 h, 48 h, 72 h, and 96 h) has potential toxicity against H. zea larvae via time- and dose-dependent manners[1].
Isochamaejasmin (40-80 μM; 24 h) causes DNA damage and increases the levels of γH2AX and OGG1 in AW1 cells. The cell cycle is arrested at the G2/M phase[1].
Isochamaejasmin (20-80 μM; 24 h) induces apoptosis of AW1 cells in a dose-dependent manner[1].
Isochamaejasmin (20-80 μM; 24 h) shows decline in the MMP, upregulation of Bax/Bcl-2 expression resulting in the release of cytochrome c into the cytosol, activation of caspase-3/9, and cleavage of PARP[1].
Isochamaejasmin shows a dose-dependent rise in the reactive oxygen species (ROS) levels, accumulation of a lipid peroxidation product, and inactivation of antioxidant enzymes in AW1 cells[1].
Isochamaejasmin induces the expression of a NF-κB-directed reporter gene in transfected HeLa cells with an EC50 of 3.23 μM. The Isochamaejasmin-stimulated NF-κB reporter activity is accompanied by nuclear translocation of NF-κB proteins and is blocked by a dominant-negative construct of IκBα. Isochamaejasmin also induces time-dependent phosphorylation of the mitogen-activated protein kinases ERK1/2 and p38, and PKCδ[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Larvae and neuronal cells (AW1)
Concentration: 6.25 μM, 12.5 μM, 25 μM, 50 μM and 100 μM
Incubation Time: 24 h, 48 h and 72 h
Result: Had potential toxicity against H. zea both in vivo and in vitro via time- and dose-dependent manners.

Cell Cycle Analysis[1]

Cell Line: Neuronal cells (AW1)
Concentration: 40 μM and 80 μM
Incubation Time: 24 h
Result: The cell cycle was arrested at the G2/M phase.

Apoptosis Analysis[1]

Cell Line: Neuronal cells (AW1)
Concentration: 20 μM, 40 μM, and 80 μM
Incubation Time: 24 h
Result: Induced apoptosis via the mitochondrial pathway in AW1 cells.

Western Blot Analysis[1]

Cell Line: Neuronal cells (AW1)
Concentration: 20 μM, 40 μM, and 80 μM
Incubation Time: 24 h
Result: Showed upregulation of Bax/Bcl-2 expression resulting in the release of cytochrome c into the cytosol, activation of caspase-3/9, and cleavage of PARP.
Molecular Weight

542.49

Formula

C30H22O10

CAS No.
SMILES

O=C1[C@]([C@]2([H])[C@@H](C3=CC=C(O)C=C3)OC4=CC(O)=CC(O)=C4C2=O)([H])[C@H](C5=CC=C(O)C=C5)OC6=CC(O)=CC(O)=C16

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Room temperature in continental US; may vary elsewhere.

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Please store the product under the recommended conditions in the Certificate of Analysis.

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Isochamaejasmin
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