Janelia Fluor® 646, Tetrazine
Based on 1 Customer Validation
Janelia Fluor 646, Tetrazine (JF646, Tetrazine) a red fluorescent dye that contains a tetrazine group. JF646, Tetrazine can be used in cellular imaging. Janelia Fluor fluorescent dyes can be chemically conjugated to the specific HaloTag substrate (a chloroalkane ligand) to form a fluorescent ligand, rather than binding directly to the HaloTag protein itself. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. Janelia Fluor 646, Tetrazine is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups (Ex/Em = 646/664 nm).
For research use only. We do not sell to patients.
- Purity: 90.17%
- CAS No.: 2042192-00-5
- Formula: C38H35N7O3Si
- Molecular Weight:665.82
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Biological Activity
Janelia Fluor fluorescent dyes do not inherently possess HaloTag-binding activity; however, reactive groups such as Tetrazine serve as reaction sites for covalent coupling-via chemical synthesis-to an amino-functionalized HaloTag chloroalkane ligand scaffold, thereby yielding a fluorescent HaloTag ligand.
JF646 can serve as ligand for self-labeling tag (such as HaloTag) [1].
JF646, Tetrazine is modified by JF646. JF646, a red fluorescent dye, is photostable, membrane-permeable, has a high extinction coefficient[2]
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
646
605
Chemical Information
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CAS No. 2042192-00-5
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Appearance Solid
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Molecular Weight 665.82
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Formula C38H35N7O3Si
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Color Pink to red
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SMILES
O=C(C1=C(C(C2=C([Si](C)(C)C3=C/4)C=C(N5CCC5)C=C2)=C3C=CC4=[N+]6CCC/6)C=C(C(NCC7=CC=C(C8=NN=CN=N8)C=C7)=O)C=C1)[O-]
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Synonyms
JF646, Tetrazine
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Grimm JB, et al. A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat Methods. 2015;12(3):244-250. doi:10.1038/nmeth.3256 [Content Brief]
[2]. Basu S, et al. FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells. Nat Commun. 2018;9(1):2520. Published 2018 Jun 28. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)