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United States

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Cat. No.: HY-D3361

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JF639-HTL

JF639-HTL is a deep-red fluorescent dye belonging to the Janelia Fluor series and also a HaloTag ligand. JF639-HTL contains a methoxy group as the R substituent, which forms a specific covalent bond with HaloTag fusion proteins. JF639-HTL is fluorogenic, exhibiting low background fluorescence in solution and a significant fluorescence enhancement upon binding to proteins. It also possesses cell permeability and excellent photostability. JF639-HTL can be used in studies related to fluorescent labeling, localization tracking, and pulse-chase imaging of proteins in living cells.

For research use only. We do not sell to patients.

Formula: C₄₃H₅₄ClNO₇Si
Molecular Weight:760.43

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

JF639-HTL Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity

Description

Chemical Information

Molecular Weight 760.43
Formula C₄₃H₅₄ClNO₇Si
SMILES

ClCCCCCCOCCOCCNC(C(C=C1)=CC(C23C4=CC=C(C5CC(OC)C5)C=C4[Si](C)(C)C6=C2C=CC(C7CC(OC)C7)=C6)=C1C(O3)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Signal Pathway Related

Transepithelial/transendothelial electrical resistance assay
TEER measures electrical resistance across epithelial or endothelial monolayers cultured on permeable supports, and the readout reflects ionic conductance through the cell barrier, especially the paracellular pathway regulated by junctional integrity. TEER can be measured without destroying the monolayer and is commonly used before or during transport, permeability, barrier-disruption, and barrier-maturation experiments. TEER values are influenced by biological maturation and technical conditions; reported factors include temperature, medium formulation, passage number, electrode geometry, membrane properties, and junctional length during early monolayer maturation. Therefore, TEER should be interpreted with blank-insert subtraction, area normalization, repeated readings, and, when possible, orthogonal barrier readouts such as FITC-dextran flux or tight-junction staining.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Walterspiel F, et al. A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells. Angewandte Chemie (International ed. in English). 2025 Dec 08;64(50):e202424955. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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