5-AF594 tyramide
Based on 1 Customer Validation
5-AF594 tyramide is a red fluorescent dye (Ex=590 nm, Em=618 nm). 5-AF594 tyramide is utilized as reporter fluorescent substrate of horseradish peroxidase (HRP)-catalyzed deposition for tyramide signal amplification (TSA). 5-AF594 tyramide can be used for multiplex immunohistochemistry (mIHC).
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.93%
- CAS 番号: 3046210-60-7
- 分子式: C43H43N3O11S2
- 分子量:841.95
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
5-AF594 tyramide assay
a. Slides are permeabilized in 0.25% Triton X-100 10 min at RT.
b. Incubate overnight at 4 °C in the primary antibody.
c. Incubate in 3% H2O2 in 1x PBS for 20 min to block endogenous peroxidase activity.
d. Detect primary antibody with anti-rabbit IgG conjugated to FITC for 1 h at RT (1: 100), and then apply an anti-FITC-conjugated HRP antibody (1: 500) overnight at 4 °C.
e. Apply a 5-AF594 tyramide for 10 min (1:200).
f. Fluorescence microscopy detection.
Note: it is suggested that the optimal concentration of 5-FITC tyramide working solution according to actual conditions.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 3046210-60-7
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性状 Solid
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分子量 841.95
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分子式 C43H43N3O11S2
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Color Dark purple to black
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SMILES
O=C(C1=C(C2=C3C=C(C(CS(=O)(O)=O)=CC(C)(C)N4C)C4=CC3=[O+]C5=CC(N(C(C)(C)C=C6CS(=O)(O)=O)C)=C6C=C25)C=CC(C(NCCC7=CC=C(C=C7)O)=O)=C1)[O-]
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
DMSO : 25 mg/mL (29.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (277 KB)
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SDS (251 KB)
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- Deutsch - DE (251 KB)
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- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1877 mL | 5.9386 mL | 11.8772 mL | 29.6930 mL |
| 5 mM | 0.2375 mL | 1.1877 mL | 2.3754 mL | 5.9386 mL | |
| 10 mM | 0.1188 mL | 0.5939 mL | 1.1877 mL | 2.9693 mL | |
| 15 mM | 0.0792 mL | 0.3959 mL | 0.7918 mL | 1.9795 mL | |
| 20 mM | 0.0594 mL | 0.2969 mL | 0.5939 mL | 1.4846 mL | |
| 25 mM | 0.0475 mL | 0.2375 mL | 0.4751 mL | 1.1877 mL |
5-AF594 tyramide 関連分類
HY-D2872 関連分類
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