安息香酸デナトニウム
Based on 2 publication(s) in Google Scholar
Denatonium benzoate (THS-839) is known as the most bitter compound and is commonly used as a deterrent (bittering agent) to activate different types of bitter taste receptors (TAS2Rs) with oral activity. Denatonium benzoate can enhance insulin secretion, has anti-inflammatory effects, and promotes allergy susceptibility .
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.87%
- CAS 番号: 3734-33-6
- 分子式: C28H34N2O3
- 分子量:446.58
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保管条件:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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HY-W011053
NeotameArtificial Sweetener -
HY-B1146R
Denatonium benzoate (Standard)Aversive Agent -
HY-100047
Nα,Nα-Bis(carboxymethyl)-L-lysineBitter taste receptors 4 Inhibitor -
HY-124956
GIV3727Bitter Taste Receptor Antagonist -
HY-100047R
Nα,Nα-Bis(carboxymethyl)-L-lysine (Standard)Bitter taste receptors 4 Inhibitor -
HY-W011053GL
Neotame (GMP Like)Artificial Sweetener -
HY-W011053R
Neotame (Standard)Standard of Neotame
MedChemExpress（MCE）の使用を引用している文献 Denatonium benzoate
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生物活性
製品説明
IC50 & Target
TAS2Rs
体外実験
Denatonium benzoate (15 μM, 5 min) reduces the spontaneous contraction activity of rat gastric smooth muscle strips[1].
Denatonium benzoate (10 μM, 24h) increases the release of β-hexosaminidase and the mobilization of Ca2+ in IgE-mediated activated RBL-2H3 and KU812 cells[2].
Denatonium benzoate (10 μM, 30 min) enhances the expression of FcεRIα on the surface of RBL-2H3 and KU812 cells[2].
Denatonium benzoate (0-25 mM, 24 h) inhibits the proliferation of RBL-2H3 and KU812 cells when above 0.1 mM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RBL-2H3, KU812
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Concentration:0, 0.01, 0.05, 0.1, 0.5, 1, 2, 2.5, 4, 5, 10, 25 mM
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Incubation Time:24 h
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Result:Showed that 0.1 mM caused significant inhibition of cell viability in RBL-2H3 and KU812 cells in a dose-dependent manner.
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Cell Line:RBL-2H3, KU812
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Concentration:10 mM
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Incubation Time:30 min
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Result:Promoted the expression of FcεRIα on the surface of mast cells.
体内実験
Denatonium benzoate (5 mg/kg, i.g., single dose) promotes allergy susceptibility in mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Rat plantar edema model[1]
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Dosage:15 mg/kg, single dose
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Administration:Intraperitoneal injection (i.p.)
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Result:Reduced foot volume and had anti-inflammatory effects.
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Animal Model:BLAB/c mice induced by OVA[2]
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Dosage:5 mg/kg, every other day, 20 days
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Administration:i.g.
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Result:Caused more severe allergic reactions and promoted allergic susceptibility in mice. Acute diarrhea began after three to four intragastric stimulations, mucus accumulation in the jejunum, β-hex and histamine levels were significantly increased, and ova in serum Specific IgE levels are also elevated.
臨床実験
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
化学情報
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CAS 番号 3734-33-6
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性状 Solid
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分子量 446.58
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分子式 C28H34N2O3
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Color White to light yellow
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SMILES
CC[N+](CC)(CC(NC1=C(C)C=CC=C1C)=O)CC2=CC=CC=C2.O=C([O-])C3=CC=CC=C3
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別名
Denatonium (benzoate); THS-839
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (2)
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Journal Impact Factor
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Most Recent
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Eur J Med Chem
2024 Dec 5:279:116842. PMID: 39260319
溶剤 & 溶解度
体外:
H2O : 20 mg/mL (44.78 mM; Need ultrasonic)
DMSO : 20 mg/mL (44.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (4.48 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 33.33 mg/mL (74.63 mM); Clear solution; Need ultrasonic
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
純度とドキュメンテーション
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データシート (284 KB)
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SDS (393 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.2392 mL | 11.1962 mL | 22.3924 mL | 55.9810 mL |
| 5 mM | 0.4478 mL | 2.2392 mL | 4.4785 mL | 11.1962 mL | |
| 10 mM | 0.2239 mL | 1.1196 mL | 2.2392 mL | 5.5981 mL | |
| 15 mM | 0.1493 mL | 0.7464 mL | 1.4928 mL | 3.7321 mL | |
| 20 mM | 0.1120 mL | 0.5598 mL | 1.1196 mL | 2.7991 mL | |
| 25 mM | 0.0896 mL | 0.4478 mL | 0.8957 mL | 2.2392 mL | |
| 30 mM | 0.0746 mL | 0.3732 mL | 0.7464 mL | 1.8660 mL | |
| 40 mM | 0.0560 mL | 0.2799 mL | 0.5598 mL | 1.3995 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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