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United States

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製品番号: HY-D3210

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DCM-KPV

DCM-KPV is a fluorescent probe targeting the human intestinal oligopeptide transporter PEPT1/SLC15A1 receptor (λ_ex=480 nm, λ_em=620-670 nm). DCM-KPV specifically binds to PepT1 via its KPV domain and mediates receptor-targeted internalization, thus effectively accumulating in the cytoplasm and nucleus of cells overexpressing this receptor. DCM-KPV has the advantages of long emission wavelength, high emission efficiency, low photobleaching, and negligible cytotoxicity. DCM-KPV maintains stable fluorescence intensity under continuous illumination, exhibiting extremely high live cell compatibility. DCM-KPV can specifically accumulate at colonic inflammatory sites through the intestinal mucosa, enabling direct non-invasive visual differentiation between chronic and acute ulcerative colitis groups and the normal group.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号: 2098632-04-1
分子式: C₄₀H₄₈N₈O₄
分子量:704.86

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

DCM-KPV 構造式

CAS 番号: 2098632-04-1

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

体外実験

DCM-KPV (1-30 μM; 24-72 h) exhibits negligible cytotoxicity and high cytocompatibility with Caco-2 cells^[2].
DCM-KPV (1.0 μM; 50 min) is efficiently internalized into Caco-2 cells via PepT1 receptor-mediated uptake, accumulating in the cytoplasm and nucleus^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[2]

Cell Line:

PepT1-overexpressed Caco-2 human colon adenocarcinoma cells
Concentration:

1-30 μM (viability assays); 1-30 μM (apoptosis assays)
Incubation Time:

24-72 h (viability assays); 24 h (apoptosis assays)
Result:

Maintained >95% cell viability at all tested concentrations and incubation times.
Showed no significant differences in cell proliferation rate or cell growth inhibition rate relative to controls after 24, 48, and 72 h of incubation.
Showed no appreciable increase in apoptosis rate relative to controls after 24 h of incubation.

体内実験

DCM-KPV (2.5 mg/kg; via anus; single dose; reserved for 1 hour) achieves noninvasive visualization of ulcerative colitis in Mus musculus (mice), with significantly higher fluorescence accumulation in chronic colitis than acute colitis and normal colon tissues, enabling discrimination between chronic, acute, and normal groups^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c mice with Ulcerative colitis (male, 6-8-week-old)^[2]
Dosage:

2.5 mg/kg
Administration:

via anus; single dose; reserved for 1 hour
Result:

Reached an average fluorescence intensity of 9.33E+08 a.u. in distal colon tissues of chronic colitis mice.
Reached an average fluorescence intensity of 4.08E+08 a.u. in distal colon tissues of acute colitis mice.
Reached an average fluorescence intensity of 2.25E+08 a.u. in distal colon tissues of normal control mice.

化学情報

CAS 番号 2098632-04-1
分子量 704.86
分子式 C₄₀H₄₈N₈O₄
SMILES

CC(C(C(N1CCN(CC1)C2=CC=C(C=C2)/C=C/C3=C/C(C4=CC=CC=C4O3)=C(C#N)/C#N)=O)NC(C5CCCN5C(C(CCCCN)N)=O)=O)C
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

取扱説明書 (2659 KB)

参考文献

[1]. Wang B, et al. A Review on Recent Advances in Peptide‐based Fluorescence Probes and their Potential Applications[J]. ChemistrySelect, 2023, 8(31): e202302216.

[2]. Zeng M, et al. Peptide Receptor-Targeted Fluorescent Probe: Visualization and Discrimination between Chronic and Acute Ulcerative Colitis. ACS Appl Mater Interfaces. 2017;9(15):13029-13036. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Minimum order quantity

100 mg

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