ヤヌスグリーンB

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Observing New Linear Structures inside Rabbit Lymphatic Vessels using Janus Green B Staining Method^[1]
(1) Preparation of experimental animals: Anesthesia is administered to 12-week-old New Zealand rabbits, and an incision is made on the left side or in the middle of the rabbit's abdomen to expose the large lymphatic vessel located next to the inferior vena cava.
(2) Dissolve Janus Green B in DMSO with a dye content of 65%.
(3) Staining: Using a 30.5-gauge needle, approximately 0.3 milliliters of the preheated Janus Green B staining solution is injected as slowly as possible into a lymph node or lymphatic vessel close to the inferior vena cava. The injection needle is gently withdrawn, and the flow of the Janus Green B staining solution in the lymphatic vessel is observed presenting a blue color.
(4) Washing and maintaining temperature: A preheated phosphate-buffered saline (pH 7.4) at 40°C is injected to remove any backflow of the Janus Green B staining solution and to maintain the tissue's temperature and active state.
(5) Observation and localization: The internal condition of the lymphatic vessels is carefully observed using a stereomicroscope to determine the location of well-stained areas. The focus of observation is adjusted according to the staining effect and individual differences.
(6) Fixing samples: The collected samples are immersed in a 10% neutral formalin solution for fixation as soon as possible.
(7) Additional staining (optional): A portion of the fixed samples is selected for secondary staining using a 0.1% (w/v) acridine orange PBS solution.
(8) Imaging: Confocal laser scanning microscopy is used for imaging analysis of the stained samples.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.