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製品番号: HY-151542

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MLKL-IN-4

MLKL-IN-4 (compound 56) is a potent MLKL (Mixed lineage kinase domain-like protein) inhibitor. MLKL-IN-4 inhibits necroptosis in HT-29 cells and acts downstream of MLKL phosphorylation, with EC₅₀ of 82 nM. MLKL-IN-4 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

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CAS 番号: 3031406-17-1
分子式: C₃₀H₂₇ClN₄O₅
分子量:559.01

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (265 KB)

MLKL-IN-4 構造式

CAS 番号: 3031406-17-1

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生物活性
- 製品説明
- Cellular Effect
- 体外実験
- 体内実験
化学情報
純度とドキュメンテーション
参考文献
Calculators
Help & FAQs
関連分類
Bioz

生物活性

製品説明

MLKL-IN-4 (compound 56) is a potent MLKL (Mixed lineage kinase domain-like protein) inhibitor. MLKL-IN-4 inhibits necroptosis in HT-29 cells and acts downstream of MLKL phosphorylation, with EC₅₀ of 82 nM^[1]. MLKL-IN-4 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

RIP kinase アイソフォーム固有の製品をすべて表示 :

Cellular Effect

Cell Line	Type	Value	Description	References
HT-29	EC₅₀	>10 μM Compound: 56	Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 5 min followed by compound washout and further induced with TSZ by celltiter-glo luminescent cell viability assay Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 5 min followed by compound washout and further induced with TSZ by celltiter-glo luminescent cell viability assay	[PMID: 36136378]
HT-29	EC₅₀	180.3 nM Compound: 56	Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 3 hr followed by compound washout and further induced with TSZ by celltiter-glo luminescent cell viability assay Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 3 hr followed by compound washout and further induced with TSZ by celltiter-glo luminescent cell viability assay	[PMID: 36136378]
HT-29	EC₅₀	188.1 nM Compound: 56	Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 3 hrs followed by TSZ stimulation by celltiter-glo luminescent cell viability assay Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 3 hrs followed by TSZ stimulation by celltiter-glo luminescent cell viability assay	[PMID: 36136378]
HT-29	EC₅₀	584.3 nM Compound: 56	Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 1 hr followed by compound washout and further induced with TSZ by celltiter-glo luminescent cell viability assay Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 1 hr followed by compound washout and further induced with TSZ by celltiter-glo luminescent cell viability assay	[PMID: 36136378]
HT-29	EC₅₀	82 nM Compound: 56	Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ (TNFalpha, Smac mimetic and z-VAD-FMK) induced necroptosis incubated for 24 hrs by celltiter-glo luminescent cell viability assay Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ (TNFalpha, Smac mimetic and z-VAD-FMK) induced necroptosis incubated for 24 hrs by celltiter-glo luminescent cell viability assay	[PMID: 36136378]

体外実験

MLKL-IN-4 (compound 56) (10 μM) slightly inhibits RIPK1, does not affect the phosphorylation status of RIPK1 and MLKL^[1].
MLKL-IN-4 (1 μM, 24 h) inhibits the translocation of MLKL to cell membranes in HT-29 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

MLKL-IN-4 (compound 56) shows T1/2 over 48 h and more than 150-fold lower reaction rates with glutathione (GSH), which potentially decreased their off-target effects and cell toxicity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

化学情報

CAS 番号 3031406-17-1
分子量 559.01
分子式 C₃₀H₂₇ClN₄O₅
SMILES

O=C(NC1=C(Cl)N(CC#CC2=CC=C(O)C(CN3C=CC=CC3=O)=C2)C(N(C)C1=O)=O)CCC4=CC=C(C)C=C4
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (265 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Cui B, et al. Discovery of a New Class of Uracil Derivatives as Potential Mixed Lineage Kinase Domain-like Protein (MLKL) Inhibitors. J Med Chem. 2022 Oct 13;65(19):12747-12780. [Content Brief]

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

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100 mg

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生物活性
- 製品説明
- 体外実験
- 体内実験
化学情報
純度とドキュメンテーション
参考文献

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MLKL-IN-4

RIP kinase アイソフォーム固有の製品をすべて表示

生物活性

化学情報

純度とドキュメンテーション

参考文献

Calculators

Help & FAQs

MLKL-IN-4 関連分類

HY-151542 関連分類