2. Epigenetics
  3. Histone Demethylase
  4. JQKD82 trihydrochloride

JQKD82 trihydrochloride  (Synonyms: JADA82 trihydrochloride; PCK82 trihydrochloride)

Cat. No.: HY-138691A Purity: 99.94%
COA Handling Instructions

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JQKD82 (JADA82) trihydrochloride is a cell-permeable and selective KDM5 inhibitor. JQKD82 trihydrochloride increases H3K4me3 and can be used for the research of multiple myeloma.

For research use only. We do not sell to patients.

JQKD82 trihydrochloride Chemical Structure

JQKD82 trihydrochloride Chemical Structure

CAS No. : 2863676-87-1

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5 mg USD 680 In-stock
10 mg USD 1100 In-stock
25 mg USD 2250 In-stock
50 mg USD 3500 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of JQKD82 trihydrochloride:

JQKD82 trihydrochloride Related Antibodies

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Description

JQKD82 (JADA82) trihydrochloride is a cell-permeable and selective KDM5 inhibitor. JQKD82 trihydrochloride increases H3K4me3 and can be used for the research of multiple myeloma[1].

IC50 & Target

KDM5

 

In Vitro

JQKD82 (0.3 μM; 24 hours) trihydrochloride causes an increase in the global H3K4me3 level of MM.1S cells[1].
? JQKD82 (0.1-10 μM; day 1-day 5) trihydrochloride inhibits the growth of MM.1S cells in a dose- and time-dependent manner. JQKD82 trihydrochloride is potent at eliciting growth suppression in MM.1S cells (IC50=0.42 μM)[1].
? JQKD82 (1 μM; 24 hours) trihydrochloride induces G1 cell-cycle arrest by 48 hours in MM.1S and MOLP-8 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

JQKD82 trihydrochloride Related Antibodies
In Vivo

JQKD82 (50-75 mg/kg; i.p.; twice a day for 3 weeks) trihydrochloride has anti-multiple myeloma activity[1].
? JQKD82 trihydrochloride displays an increase in H3K4me3 levels and results in a dramatic reduction of MYC immuno-staining in vivo[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Six-week-old female NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice (bearing MOLP-8 TurboGFP-Luc cells)[1]
Dosage: 50 mg/kg, 75 mg/kg
Administration: i.p.; twice a day for 3 weeks
Result: Significantly reduced tumor burden.
Molecular Weight

610.01

Formula

C27H43Cl3N4O5
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C(C1=CC(CNCC(N(CCN(C)C)CC)=O)=NC=C1)OC2=CC=C(C=C2OC(C)C)OC(C)C.[H]Cl.[H]Cl.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (81.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6393 mL 8.1966 mL 16.3932 mL
5 mM 0.3279 mL 1.6393 mL 3.2786 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1 mg/mL (1.64 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1 mg/mL (1.64 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.94%

COA (245 KB) HNMR (275 KB) LCMS (264 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6393 mL 8.1966 mL 16.3932 mL 40.9829 mL
5 mM 0.3279 mL 1.6393 mL 3.2786 mL 8.1966 mL
10 mM 0.1639 mL 0.8197 mL 1.6393 mL 4.0983 mL
15 mM 0.1093 mL 0.5464 mL 1.0929 mL 2.7322 mL
20 mM 0.0820 mL 0.4098 mL 0.8197 mL 2.0491 mL
25 mM 0.0656 mL 0.3279 mL 0.6557 mL 1.6393 mL
30 mM 0.0546 mL 0.2732 mL 0.5464 mL 1.3661 mL
40 mM 0.0410 mL 0.2049 mL 0.4098 mL 1.0246 mL
50 mM 0.0328 mL 0.1639 mL 0.3279 mL 0.8197 mL
60 mM 0.0273 mL 0.1366 mL 0.2732 mL 0.6830 mL
80 mM 0.0205 mL 0.1025 mL 0.2049 mL 0.5123 mL
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JQKD82 trihydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

JQKD82 trihydrochloride2863676-87-1JADA82 trihydrochloridePCK82 trihydrochlorideHistone DemethylaseMM.1ScellsKDM5histoneH3lysinecancersInhibitorinhibitorinhibit

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