2. Cytoskeleton Stem Cell/Wnt
  3. Annexin A YAP
  4. PY-60

PY-60 is a robust and specific activator of YAP transcriptional activity that targets annexin A2 (ANXA2) with a Kd of 1.4 µM. PY-60 directly binds to ANXA2 and antagonizes its normal cellular function of repressing YAP activity.

For research use only. We do not sell to patients.

CAS No. : 2765218-56-0

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Based on 16 publication(s) in Google Scholar

PY-60 Related Antibodies

Top Publications Citing Use of Products

    PY-60 purchased from MedChemExpress. Usage Cited in: Biomaterials. 2024 Jul:308:122542.

    YAP activity ratio for NIH 3T3 cells treated with inhibitors, one-way ANOVA with multiple comparisons, n=6. The results showed that PY-60 (10 μM; 72 h) significantly increased the YAP activity ratio.

    PY-60 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Nov 20:225:333-345.  [Abstract]

    Representative Western blot bands. A total 5.0 μl of PBS, DMSO, U-46619 (0.1 μg/kg) or PY-60 (0.2 mg/kg) were administered via the intracerebroventricular (i.c.v) route at 1.5 h after SAH.

    PY-60 purchased from MedChemExpress. Usage Cited in: Transl Res. 2024 Jun:268:63-78.  [Abstract]

    Confocal images of YAP after different treatments for 24 h in H357 Cis R cells, Scale bar = 10 μM. Blue signals indicate cell nuclei stained by DAPI and red indicates the YAP expression. Immunofluorescence was performed in cells from different treatment groups (2.5 μM cisplatin, 10 μM PY-60, 2.5 μM cisplatin + 5 μM PL, 2.5 μM cisplatin + 5 μM smart PL-NPs, 10 μM PY-60 + 2.5 μM cisplatin + 5 μM PL, and 10 μM PY-60 + 2.5 μM cisplatin + 5 μM smart PL-NPs) for 24 h. The primary antibody used was rabbit monoclonal anti-YAP.

    PY-60 purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2024 Aug;57(8):e13640.  [Abstract]

    Relative mRNA expression levels of M1/M2-associated genes after DMSO, GsMTx4, GsMTx4+PY-60 (10 μM; 12 h) treatment when cultured on stiff substrates (*P<0.05, **P<0.01).

    PY-60 purchased from MedChemExpress. Usage Cited in: J Bone Miner Metab. 2022 Nov;40(6):890-899.  [Abstract]

    PY-60 (10 μM, applied topically to the skin on the back of mice for 10 consecutive days).
    IHC analysis was done to detect changes in YAP, Ki67, MMP2, MMP9, MMP14 and cleaved caspase three expressions in indicated groups. **P < 0.01

    PY-60 purchased from MedChemExpress. Usage Cited in: J Bone Miner Metab. 2022 Nov;40(6):890-899.  [Abstract]

    Cells transfected with pcDNA3.1 or pcDNA3.1/LINC01089 were injected into mice, and 10 µM PY-60 was applied topically to the dorsal skin of mice over the course of 10 days. Tumor volume was measured in each indicated group every 3 days from day 7, and after all mice were sacrificed at day 28, tumor weight was examined.

    View All Annexin A Isoform Specific Products:

    View All Isoforms
    AnxA1 AnxA3 AnxA4 AnxA6 AnxA7 AnxA8 AnxA9 AnxA10 AnxA11 AnxA13

    View All YAP Isoform Specific Products:

    View All Isoforms
    YAP/TAZ YAP/TAZ-TEAD TEAD YAP1

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    PY-60 is a robust and specific activator of YAP transcriptional activity that targets annexin A2 (ANXA2) with a Kd of 1.4 µM. PY-60 directly binds to ANXA2 and antagonizes its normal cellular function of repressing YAP activity[1].

    In Vitro

    PY-60 targets ANXA2 to activate YAP[1].
    PY-60, a thiazole-substituted derivative, dose-dependently induces luciferase activity in 293A-TEAD-LUC cells in the presence or absence of serum when cells were plated at high cell density (EC50= 1.5 and 1.6 μM, respectively). PY-60 treatment also dose-dependently promoted the association of YAP and TEAD proteins in cells and induced the nuclear localization of YAP in response to increased cell density. PY-60 robustly increases the levels of YAP-controlled transcripts (that is, ANRKD1, CYR61 and CTGF) in 293A cells and other human cell lines (that is, MCF10A, HEK293T, H69 and HaCaT), but did not augment the levels of YAP itself (YAP1)[1].
    PY-60 activates a proproliferative, YAP-dependent transcriptional program in the adult animal capable of remodeling the epidermis through proliferation[1].
    PY-60 liberates the ANXA2-YAP complex from the cell membrane and competes for ANXA2 binding of phosphoinositides[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    PY-60 Related Antibodies
    In Vivo

    PY-60 (10 uM; applied topically to the dorsal skin of wild-type adult C57BL/6 mice over the course of 10 days) promotes a dramatic expansion of keratinocytes and K14-positive precursors, as assessed by hematoxylin and eosin and anti-K14 histological staining at the study end. PY-60 results in an approximate doubling of epidermal thickness, a result derived from an increased number of keratinocytes per unit length of skin[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    313.37

    Formula

    C16H15N3O2S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(C1=NOC(C2=CC=CC=C2)=C1)NCCCC3=NC=CS3
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (319.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1911 mL 15.9556 mL 31.9112 mL
    5 mM 0.6382 mL 3.1911 mL 6.3822 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (7.98 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.42%

    SDS (251 KB)

    COA (246 KB) HNMR (354 KB) RP-HPLC (254 KB) LCMS (95 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.1911 mL 15.9556 mL 31.9112 mL 79.7779 mL
    5 mM 0.6382 mL 3.1911 mL 6.3822 mL 15.9556 mL
    10 mM 0.3191 mL 1.5956 mL 3.1911 mL 7.9778 mL
    15 mM 0.2127 mL 1.0637 mL 2.1274 mL 5.3185 mL
    20 mM 0.1596 mL 0.7978 mL 1.5956 mL 3.9889 mL
    25 mM 0.1276 mL 0.6382 mL 1.2764 mL 3.1911 mL
    30 mM 0.1064 mL 0.5319 mL 1.0637 mL 2.6593 mL
    40 mM 0.0798 mL 0.3989 mL 0.7978 mL 1.9944 mL
    50 mM 0.0638 mL 0.3191 mL 0.6382 mL 1.5956 mL
    60 mM 0.0532 mL 0.2659 mL 0.5319 mL 1.3296 mL
    80 mM 0.0399 mL 0.1994 mL 0.3989 mL 0.9972 mL
    100 mM 0.0319 mL 0.1596 mL 0.3191 mL 0.7978 mL
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    PY-60 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    PY-602765218-56-0PY60PY 60Annexin AYAPAnxAYes-associated proteintranscriptionalannexinA2C57BL/6miceskinepidermisproliferationInhibitorinhibitorinhibit

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