Ac-DNLD-AMC
Ac-WLA-AMC is a fluorogenic substrate of caspase-3. Ac-WLA-AMC is cleaved to release the fluorescent moiety 7-amino-4-methylcoumarin (AMC), which can be used to quantify the β5c subunit activity.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- CAS No.: 958001-92-8
- 화학식: C30H38N6O12
- 분자량:674.66
-
보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocol
- 순도&문서
- References
-
Help & FAQs
-
HY-P2208
Z-IETD-AFCCaspase-8 Fluorescence Substrate -
HY-123053
Z-Leu-Leu-Glu-AMCPeptide-AMC Linked Substrate -
HY-P2620
Ac-LETD-AFCCaspase-8 Fluorogenic Substrate -
HY-P1005
Ac-DEVD-AFCCaspase-3 Fluorogenic Substrate -
HY-P1003
Ac-DEVD-AMCCaspase-3/7 Fluorescent Substrate -
HY-W011063
Gly-Phe-β-naphthylamideCathepsin C Substrate -
HY-D1652
Ac-LEHD-AMCCaspase-9 Fluorogenic Substrate -
HY-130412
FlAsH-EDT2Protein Labeling Reagent -
HY-113953
Z-Asp-CH2-DCBCaspase Inhibitor -
HY-D1176
Calcium Green 1AMCalcium Indicator -
HY-D0121A
INDO 1 pentapotassiumCa2+ Indicator -
HY-P2614
Mca-DEVDAP-K(Dnp)-OHCaspase-3 Fluorogenic Substrate
Biological Activity
제품 설명
In Vitro
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of caspase[1]:
1.Cells were harvested and washed with PBS.
2.After centrifugation, they were resuspended in PBS at a concentration of 2 × 106 cells/100 μL.
3.25 μL of the suspension was added to a 96-well plate and mixed with the appropriate peptide substrate dissolved in a standard reaction buffer (100 mM HEPES, 10 % sucrose, 5 mM DTT, 0.001 % NP-40, and 0.1 % CHAPS, pH 7.2).
4.Ac-DNLD-AMC was monitored by AMC liberation in a multimode detector using 380-nm excitation and 460-nm emission wavelengths.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Emission (Em)
445
Excitation (Ex)
360
Chemical Information
-
CAS No. 958001-92-8
-
분자량 674.66
-
화학식 C30H38N6O12
-
선적
Room temperature in continental US; may vary elsewhere.
-
보관
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocol
-
Apoptosis Solutions
Apoptosis is a regulated, generally non-lytic cell-death pathway that removes unwanted, damaged, infected, or abnormal cells through coordinated morphological changes, caspase activation, DNA fragmentation, and membrane remodeling. The intrinsic apoptosis pathway is controlled mainly by mitochondrial outer membrane permeabilization, BCL-2 family proteins, cytochrome c release, apoptosome formation, caspase-9 activation, and downstream executioner caspase-3/7 activation. The extrinsic apoptosis pathway is initiated by death receptors such as Fas, TNFR, and TRAIL receptors, which recruit adaptor proteins and activate caspase-8 before engaging executioner caspases or mitochondrial amplification through BID cleavage. Apoptosis is linked to many phenotypes, including cancer cell killing, tissue homeostasis, immune regulation, neurodegeneration, infection response, and treatment-induced cytotoxicity; unresolved questions include how apoptosis interacts with necroptosis, pyroptosis, ferroptos
순도&문서
References
[1]. Kulikov AV, et al. Targeting mitochondria by α-tocopheryl succinate overcomes hypoxia-mediated tumor cell resistance to treatment. Cell Mol Life Sci. 2014 Jun;71(12):2325-33. [Content Brief]
[2]. Yoshida K, et al. Caspase-independent cell death without generation of reactive oxygen species in irradiated MOLT-4 human leukemia cells. Cell Immunol. 2009;255(1-2):61-8. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Ac-DNLD-AMC Related Classifications
HY-P3110 Related Classifications
Powered by Bioz