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Cat. No.: HY-D3185

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AlDeSense AM

AlDeSense AM is a cell-permeable ALDH1A1-selective fluorescent reporter. AlDeSense AM is oxidized by ALDH1A1, which eliminates photoinduced electron transfer quenching and enhances the fluorescent signal. AlDeSense AM can be used to detect cells with cancer stem cell properties, as well as to monitor the plasticity of cancer stem cells in cell culture systems and animal models. AlDeSense AM is applicable to the study of cancers associated with cancer stem cells, including chronic myeloid leukemia, melanoma, and breast cancer.

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CAS No.: 2243148-62-9
화학식: C₂₄H₁₆F₂O₆
분자량:438.38

보관:

Please store the product under the recommended conditions in the Certificate of Analysis.

AlDeSense AM Chemical Structure

CAS No.: 2243148-62-9

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- In Vitro
- In Vivo
Chemical Information
순도&문서
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

All Aldehyde Dehydrogenase (ALDH) Isoforms :

In Vitro

AlDeSense AM (1.5-2 μM; 30 min) selectively labels human chronic myeloid leukemia K562 cells with active ALDH1A1, which correspond to the CD34⁺/CD38⁻/CD133⁺leukemia stem cell population^[1].
AlDeSense AM (2 μM; 1 h) detects elevated ALDH1A1 activity in MDA-MB-231 breast cancer mammospheres (cancer stem cell-enriched), and the fluorescent signal decreases as the mammospheres differentiate over 36 h^[1].
The fluorescence signal of AlDeSense AM (2 μM; 1 h) in CSC-enriched B16F0 e-CSC is 11.3-fold higher than that in non-CSC B16F0 cells, confirming elevated ALDH1A1 activity in e-CSC^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[1]

Cell Line:

K562 human chronic myeloid leukemia cells
Concentration:

1.5 μM (flow cytometry staining); 2 μM (confocal imaging staining)
Incubation Time:

30 min at room temperature (confocal imaging staining)
Result:

Identified a population of brightly fluorescent cells corresponding to the CD34+/CD38-/CD133+ leukemic stem cell profile.
Showed a small population of highly fluorescent cells via confocal imaging.
Completely abolished the fluorescence signal after ALDH1A1 siRNA knockdown.

Immunofluorescence^[1]

Cell Line:

MDA-MB-231 breast cancer mammospheres (CSC-enriched) and differentiating mammospheres
Concentration:

2 μM
Incubation Time:

1 h at room temperature (at 1, 6, 12, 24, 36 h time points during differentiation)
Result:

Exhibited 3-fold brighter fluorescence in freshly isolated mammospheres compared to control-stained mammospheres.
Gradually decreased fluorescence signal over 36 h of differentiation.

Immunofluorescence^[1]

Cell Line:

B16F0 murine melanoma e-CSCs (CSC-enriched via spiral-patterned hydrogel culture) and non-CSCs (standard culture)
Concentration:

2 μM
Incubation Time:

1 h at room temperature
Result:

Showed 11.3-fold brighter fluorescence in e-CSCs than in non-CSCs.
Showed 9.0-fold brighter fluorescence in e-CSCs than in e-CSCs stained with control reagent.
Colocalized with the CD271 melanoma stem cell marker in e-CSCs.

In Vivo

AlDeSense AM (15 μM; lung perfusion; single dose) selectively detects elevated ALDH1A1 activity in e-CSC-derived lung metastases in C57BL/6J mice, producing a significantly higher fluorescence signal than in non-CSC-derived metastases^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6J (female, 6 to 8 weeks old)^[1]
Dosage:

15 μM
Administration:

lung perfusion; single dose
Result:

Produced a mean fluorescence signal of ~1×10¹¹ a.u.
in lungs with e-CSC metastases.
Showed a significantly higher mean fluorescence signal (~1×10¹¹ a.u.) than the ~7×10¹⁰ a.u.
measured in lungs with non-CSC metastases.
Demonstrated significantly higher fluorescence in e-CSC metastases than e-CSC metastases treated with Ctrl-AlDeSense AM.

Chemical Information

CAS No. 2243148-62-9
분자량 438.38
화학식 C₂₄H₁₆F₂O₆
SMILES

CC(OCOC1=C(C=C2C(OC3=CC(C(F)=CC3=C2C4=CC=C(C=C4C)C=O)=O)=C1)F)=O
선적

Room temperature in continental US; may vary elsewhere.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

순도&문서

Handling Instructions (2659 KB)

References

[1]. Anorma C, et al. Surveillance of Cancer Stem Cell Plasticity Using an Isoform-Selective Fluorescent Probe for Aldehyde Dehydrogenase 1A1. ACS Cent Sci. 2018 Aug 22;4(8):1045-1055. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

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100 mg

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Biological Activity
- 제품 설명
- In Vitro
- In Vivo
Chemical Information
순도&문서
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AlDeSense AM

All Aldehyde Dehydrogenase (ALDH) Isoforms

Biological Activity

Chemical Information

순도&문서

References

Calculators

Help & FAQs

AlDeSense AM Related Classifications

HY-D3185 Related Classifications