BODIPY FL-C16
Based on 1 Customer Validation
BODIPY FL-C16 is a BODIPY-labeled analog of Palmitic acid (HY-N0830), which serves as a fluorescent lipid tracer. BODIPY FL-C16 also acts as a ligand for liver fatty acid-binding protein (L-FABP)
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- Purity: 99.84%
- CAS No.: 158757-82-5
- 화학식: C27H41BF2N2O2
- 분자량:474.43
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보관:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
BODIPY FL-C16 (1-7 μM; 15 min-5 h pulse, up to 24 h chase) metabolically labels a homogeneous subpopulation of ~80 nm small extracellular vesicles of endosomal/MVB origin in human Mel501, Me665/1, A375/M, HEK293T, and HeLa cells, with maximal secretion plateauing 6 h after a 2 h pulse, and the labelled vesicles showing high enrichment for CD63, moderate enrichment for CD81, and low enrichment for CD9[1].
BODIPY FL-C16 (0.5 μM; 5 μM L-FABP/I-FABP) has a low fluorescence quantum yield of 0.03 in PBS-CGM buffer, a high quantum yield of ~0.93 in methanol, and displays increased quantum yields of ~0.87 and ~0.72 when bound to recombinant rat L-FABP and I-FABP, respectively[2].
BODIPY FL-C16 (0.5 μM; ~1 min) binds to recombinant rat L-FABP with an apparent KD of 270 nM and to recombinant rat I-FABP with an apparent KD of 330 nM, occupying the fatty acid-binding site on I-FABP and one of two fatty acid-binding sites on L-FABP[2].
BODIPY FL-C16 (5 μM; 15 min at 37°C) extensively co-localizes with mitochondria and endoplasmic reticulum/Golgi, but not lysosomes, in fully differentiated human intestinal Caco-2 cells[2].
BODIPY FL-C16 (5 μM; 15 min at 37°C) is not esterified or incorporated into complex lipids in fully differentiated human intestinal Caco-2 cells within a 15-minute incubation period at 37°C[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 158757-82-5
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Appearance Solid
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분자량 474.43
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화학식 C27H41BF2N2O2
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Color Orange to red
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SMILES
F[B-]([N+]1=C(CCCCCCCCCCCCCCCC(O)=O)C=CC1=C2)(F)N3C2=C(C)C=C3C
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선적
Room temperature in continental US; may vary elsewhere.
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보관
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
용액&용해도
DMSO : 50 mg/mL (105.39 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Barreca V, et al. Metabolic labelling of a subpopulation of small extracellular vesicles using a fluorescent palmitic acid analogue. J Extracell Vesicles. 2023;12(12):e12392. [Content Brief]
[2]. Thumser AE, et al. Characterization of a BODIPY-labeled fluorescent fatty acid analogue. Binding to fatty acid-binding proteins, intracellular localization, and metabolism. Mol Cell Biochem. 2007;299(1-2):67-73. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1078 mL | 10.5390 mL | 21.0779 mL | 52.6948 mL |
| 5 mM | 0.4216 mL | 2.1078 mL | 4.2156 mL | 10.5390 mL | |
| 10 mM | 0.2108 mL | 1.0539 mL | 2.1078 mL | 5.2695 mL | |
| 15 mM | 0.1405 mL | 0.7026 mL | 1.4052 mL | 3.5130 mL | |
| 20 mM | 0.1054 mL | 0.5269 mL | 1.0539 mL | 2.6347 mL | |
| 25 mM | 0.0843 mL | 0.4216 mL | 0.8431 mL | 2.1078 mL | |
| 30 mM | 0.0703 mL | 0.3513 mL | 0.7026 mL | 1.7565 mL | |
| 40 mM | 0.0527 mL | 0.2635 mL | 0.5269 mL | 1.3174 mL | |
| 50 mM | 0.0422 mL | 0.2108 mL | 0.4216 mL | 1.0539 mL | |
| 60 mM | 0.0351 mL | 0.1756 mL | 0.3513 mL | 0.8782 mL | |
| 80 mM | 0.0263 mL | 0.1317 mL | 0.2635 mL | 0.6587 mL | |
| 100 mM | 0.0211 mL | 0.1054 mL | 0.2108 mL | 0.5269 mL |
BODIPY FL-C16 Related Classifications
HY-D1736 Related Classifications
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