Coppersensor-3

Coppersensor-3 (CS3) is a visible-wavelength Cu (I) sensor that can serve as a fluorescent probe, λ_abs 540 nm, λ_em 548 nm, 75-fold turn-on, Φ = 0.40, K_d = 9 × 10^-14 M^[1].
Coppersensor-3 reversibly senses Cu (I) in the presence of GSH, BSA, and DMPC, while maintaining Cu (I) responsiveness over the pH range of 3-8^[1].
Coppersensor-3 enables screening for ATP7B-dependent lysosomal copper import in hepatocytes^[1].
Coppersensor-3 detects copper hyperaccumulation in zinc-deficient Chlamydomonas reinhardtii and requires CRR1^[1].
Coppersensor-3 detects basal labile Cu (I) in HEK293T cells and reports copper that is depletable by BCS; imaging results report KCl depolarization-induced, calcium-dependent copper transport from the soma to dendritic processes of rat hippocampal neurons; reveals copper-rich endosomal compartments in CTR2^−/− mouse fibroblasts, whose number is reduced by CTR1 truncation; detects changes in copper status in human epithelial cell types and is unaffected by cisplatin^[1][2].
Coppersensor-3 (CS3) (2 μM) exhibits time-dependent fluorescence enhancement in the DMPC-DMPG liposome model membrane system, a phenomenon arising from the slow dissolution of colloidal aggregates into the lipid bilayer^[3].
Coppersensor-3 (CS3) (2 μM; 10 min) can be used to determine that CTR1 is required for bacterial infection-induced cytosolic copper accumulation^[4].