Biological Activity
제품 설명
Chemical Information
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CAS No. 544-04-7
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분자량 163.13
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화학식 C8H5NO3
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SMILES
O=C(/C=C/C#CC#CC(N)=O)O
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Synonyms
Diatretyne amide
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Structure Classification
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Initial Source
C.diatreta
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocol
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Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
순도&문서
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)