Oxyfluorfen
Based on 1 Customer Validation
Oxyfluorfen is a pre- and post-emergence diphenyl ether herbicide to control annual broad-leaved and grass weeds. Oxyfluorfen is a protoporphyrinogen oxidase inhibitor and inhibits photosynthesis by blocking chlorophyll synthesis. Oxyfluorfen can inhibit cell growth. Oxyfluorfen induces DNA damage and exhibits toxicity toward aquatic organisms such as Paramisgurnus dabryanus. Oxyfluorfen has genome-level deleterious effects on fish that can lead to stunted skeletal growth. Oxyfluorfen induces transverse limb deficiency or craniosynostosis.
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- Purity : 99.69%
- CAS No.: 42874-03-3
- 화학식: C15H11ClF3NO4
- 분자량:361.70
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
In Vitro
Oxyfluorfen (10 nM, 12 d) strongly inhibits the growth of normal soybean cells but not resistant cells[2].
Oxyfluorfen (0-100 μM) inhibits the total extractable Protox activity in normal cells but not resistant cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
Oxyfluorfen (0-800 ppm, in the diet, 7 or 28 d) exhibits liver toxicity in mice[4].
Oxyfluorfen (40-1600 ppm, in the diet, 3 or 10 d) results in a dose-responsive induction in PPARα genes (Cyp4a10, Acox1, Cte-1 and Pmp-70) in mice model[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male CD-1 mice (up to 12 weeks) with ad libitum access to feed[4]
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Dosage:0, 40, 200 and 800 ppm
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Administration:Added to diet for 7 or 28 days
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Result:Resulted in significant increases in absolute and relative lever weights with 800 ppm.
Increased the liver weights with no statistical significance at 200 ppm.
Increased peroxisomal ACO activity 1.5- and 5-fold above control levels after 28 days with 200 and 800 ppm, respectively.
Increased cell proliferation after 28 days with 800 ppm.
Led to hypertrophy, vacuolization, single-cell necrosis and additional liver changes in mice given 800 ppm for 28 days.
Chemical Information
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CAS No. 42874-03-3
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Appearance Solid
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분자량 361.70
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화학식 C15H11ClF3NO4
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Color Off-white to light yellow
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SMILES
FC(C1=CC=C(OC2=CC=C([N+]([O-])=O)C(OCC)=C2)C(Cl)=C1)(F)F
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
In Vitro:
DMSO : 100 mg/mL (276.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
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Protocol for Cell Counting and Cell Density Analysis
Cell counting and cell-density analysis estimate the number of cells in a known volume or field area. Manual hemocytometer counting uses a chamber of defined geometry to convert counted cells into cells/mL, while automated counters and image-analysis workflows detect cell objects from optical, brightfield, fluorescence, impedance, or digital-image features. Trypan blue viability counting is based on dye exclusion: viable cells with intact membranes exclude dye, while non-viable cells with compromised membranes stain blue. The readout is total cell density, viable-cell density, dead-cell density, and percent viability. Cell density can also be estimated from microscopy images by counting objects per image area, from flow cytometry using calibrated volume or reference particles, or from in situ microscopy in bioreactors after calibration against reference methods such as hemocytometer or flow cytometry.
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Protocol for Fluorescence In Situ Hybridization (FISH)
Fluorescence in situ hybridization detects specific DNA or RNA sequences inside fixed cells or tissue sections by hybridizing fluorescently labeled nucleic-acid probes to complementary target sequences, allowing the target’s copy number, chromosomal position, spatial distribution, or transcript abundance to be visualized microscopically. DNA-FISH detects genomic loci, chromosomal gains/losses, amplifications, deletions, and rearrangements, while RNA-FISH detects RNA molecules or transcript localization; in cancer cells, mouse tumors, neurons, organoids, macrophages, or drug-screening samples, the readout is fluorescent puncta, fusion/split signals, or localized RNA signal interpreted relative to validated controls.
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Somatic Cell Culture
A method of simulating the in vivo environment in vitro to maintain the cell growth, differentation and main functions.
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CCK-8/WST-8 Cell Proliferation Assay
The CCK-8/WST-8 assay is based on the reduction of the water-soluble tetrazolium salt WST-8 to a water-soluble formazan product by cellular dehydrogenases in metabolically active cells, where the generated formazan amount is proportional to the number of living cells and is quantified by measuring absorbance in the visible range, providing a colorimetric readout for cell viability and proliferation assessment. This class of tetrazolium-based assays improves upon earlier MTT-based systems by producing a water-soluble formazan, eliminating the need for organic solubilization steps and enabling direct spectrophotometric measurement in culture medium.
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Cell Counting-Based Growth Curve Assay
Cell counting-based growth curve assays quantify cell proliferation by directly measuring changes in viable cell number over time using manual or automated counting methods such as hemocytometer-based counting or instrument-assisted cell enumeration, enabling construction of growth curves that reflect population expansion dynamics in response to culture conditions. A widely used approach is trypan blue exclusion with hemocytometer counting, where membrane-compromised (non-viable) cells take up the dye, allowing discrimination between viable and non-viable cells while simultaneously enabling total cell number quantification. Repeated sampling across time points allows estimation of proliferation rate, growth phases, and comparative growth kinetics between experimental conditions.
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MTT Cell Proliferation Assay
The MTT assay is a colorimetric endpoint assay for estimating viable cell number, cell growth, cytotoxicity, or cell activation in cultured mammalian cells. Living cells reduce the yellow tetrazolium salt MTT into purple/blue formazan, while dead cells do not generate the same signal; the resulting color can be quantified with a multiwell spectrophotometer. MTT reduction is commonly interpreted as a readout of metabolic activity that often correlates with viable cell number, but it should not be treated as a direct cell-counting method unless the assay is optimized for the cell type and experimental condition. Studies show that MTT reduction can involve mitochondrial and non-mitochondrial reducing systems, and formazan may accumulate in intracellular lipid droplets rather than simply marking mitochondria.
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Genotoxicity/Mutagenicity Study
The bacterial reverse mutation assay detects point mutations that restore amino-acid prototrophy in auxotrophic Salmonella typhimurium or Escherichia coli tester strains; after exposure to a test article, mutagenic activity is read out as an increased number of revertant colonies on minimal agar compared with the vehicle control. The assay uses tester strains with different mutation targets so that base-substitution and frameshift mutagens can be detected, and testing is performed with and without exogenous mammalian metabolic activation because some chemicals require biotransformation to become mutagenic.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
순도&문서
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Data Sheet (275 KB)
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SDS (458 KB)
- English - EN (458 KB)
- Français - FR (458 KB)
- Deutsch - DE (458 KB)
- Norwegian - NO (458 KB)
- Español - ES (458 KB)
- Swedish - SV (458 KB)
- Italian - IT (458 KB)
- Korean - KR (458 KB)
- Portuguese - PT (458 KB)
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Handling Instructions (2659 KB)
References
[1]. Chi Wu, et al. Sorption, Degradation and Bioavailability of Oxyfluorfen in Biochar-Amended Soils. Sci Total Environ. 2019 Mar 25;658:87-94. [Content Brief]
[2]. E Warabi, et al. Resistance of a Soybean Cell Line to Oxyfluorfen by Overproduction of Mitochondrial Protoporphyrinogen Oxidase. Pest Manag Sci. 2001 Aug;57(8):743-8. [Content Brief]
[4]. Stagg, N. J., et al., (2012). Assessment of possible carcinogenicity of oxyfluorfen to humans using mode of action analysis of rodent liver effects. Toxicological sciences: an official journal of the Society of Toxicology, 128(2), 334–345. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7647 mL | 13.8236 mL | 27.6472 mL | 69.1181 mL |
| 5 mM | 0.5529 mL | 2.7647 mL | 5.5294 mL | 13.8236 mL | |
| 10 mM | 0.2765 mL | 1.3824 mL | 2.7647 mL | 6.9118 mL | |
| 15 mM | 0.1843 mL | 0.9216 mL | 1.8431 mL | 4.6079 mL | |
| 20 mM | 0.1382 mL | 0.6912 mL | 1.3824 mL | 3.4559 mL | |
| 25 mM | 0.1106 mL | 0.5529 mL | 1.1059 mL | 2.7647 mL | |
| 30 mM | 0.0922 mL | 0.4608 mL | 0.9216 mL | 2.3039 mL | |
| 40 mM | 0.0691 mL | 0.3456 mL | 0.6912 mL | 1.7280 mL | |
| 50 mM | 0.0553 mL | 0.2765 mL | 0.5529 mL | 1.3824 mL | |
| 60 mM | 0.0461 mL | 0.2304 mL | 0.4608 mL | 1.1520 mL | |
| 80 mM | 0.0346 mL | 0.1728 mL | 0.3456 mL | 0.8640 mL | |
| 100 mM | 0.0276 mL | 0.1382 mL | 0.2765 mL | 0.6912 mL |
Oxyfluorfen Related Classifications
HY-119176 Related Classifications
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