Lactokinin 

Lactokinin is an Endothelin-1 (HY-P0202) release inhibitor and ACE inhibitor, with an ACE IC₅₀ value of 29 μM. Lactokinin binds to bovine β-lactoglobulin variant A to form a non-covalent complex, which delays its hydrolysis by chymotrypsin, as well as by pepsin/chymotrypsin, in vitro. Lactokinin can be used in research related to hypertension^[1][2][3].

Lactokinin is bound to purified bovine β-lactoglobulin variant A via non-covalent interactions, and the bound peptide is protected from oxidation and ion adduction during ionization compared to free lactokinin^[1].
Lactokinin (0.1-1 mM) inhibits basal and thrombin-stimulated endothelin-1 release from cultured porcine aortic endothelial cells, with 1 mM reducing basal release by 29% and 0.1 mM and 1 mM reducing thrombin-stimulated release by 32% and 43%, respectively^[2].
Lactokinin inhibits porcine kidney angiotensin-converting enzyme activity with an IC₅₀ of 29 μM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

837.04

C₃₇H₆₄N₁₂O₈S

132160-04-4

Ala-Leu-Pro-Met-His-Ile-Arg

ALPMHIR

C[C@H](N)C(N[C@@H](CC(C)C)C(N1[C@@H](CCC1)C(N[C@@H](CCSC)C(N[C@H](C(N[C@@H]([C@@H](C)CC)C(N[C@H](C(O)=O)CCCNC(N)=N)=O)=O)CC2=CN=CN2)=O)=O)=O)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Roufik S, et al. Physicochemical characterization and in vitro digestibility of β-lactoglobulin/β-Lg f142-148 complexes[J]. International dairy journal, 2007, 17(5): 471-480.

  [2]. Maes W, et al. Influence of the lactokinin Ala-Leu-Pro-Met-His-Ile-Arg (ALPMHIR) on the release of endothelin-1 by endothelial cells. Regulatory peptides. 2004 Apr 15;118(1-2):105-9.  [Content Brief]

  [3]. Pina AS, et al. Studies on the molecular recognition between bioactive peptides and angiotensin-converting enzyme. Journal of molecular recognition : JMR. 2009;22(2):162-8.